Reference : Mitochondrial DNA haplogroups modulate the serum levels of biomarkers in patients wit...
Scientific journals : Article
Human health sciences : Rheumatology
http://hdl.handle.net/2268/75196
Mitochondrial DNA haplogroups modulate the serum levels of biomarkers in patients with osteoarthritis.
English
Rego-Perez, I. [> > > >]
Fernandez-Moreno, M. [> > > >]
Deberg, Michelle mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
Pertega, S. [> > > >]
Fenandez-Lopez, C. [> > > >]
Oreiro, N. [> > > >]
Henrotin, Yves mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) - Didactique des sciences de la santé - Pathologie générale et physiopathologie >]
Blanco, F. J. [> > > >]
2010
Annals of the Rheumatic Diseases
BMJ Group
69
5
910-7
Yes (verified by ORBi)
International
0003-4967
1468-2060
London
United Kingdom
[en] Aged ; Biological Markers/blood ; Collagen Type II/blood ; DNA, Mitochondrial/genetics ; Female ; Haplotypes ; Humans ; Hyaluronic Acid/blood ; Male ; Middle Aged ; Osteoarthritis, Hip/blood/genetics/radiography ; Osteoarthritis, Knee/blood/genetics/radiography ; Peptide Fragments/blood ; Retrospective Studies ; Severity of Illness Index
[en] OBJECTIVE: To analyse the influence of mitochondrial DNA (mtDNA) haplogroups on serum levels of molecular biomarkers in patients with osteoarthritis (OA). METHODS: Serum levels of molecular biomarkers of cartilage metabolism (collagen type II markers: C-terminal neoepitope generated by the collagenase-mediated cleavage of collagen type II triple helix (C2C), collagen type II (Coll2-1, and its nitrated form, Coll2-1NO(2)), procollagen type II (CPII)), synovial metabolism (hyaluronic acid (HA)) and cartilage and synovial turnover (cartilage glycoprotein 39 (YKL-40)) were analysed in 73 patients with OA and 77 healthy controls using ELISAs. All participants had been previously genotyped for the mtDNA haplogroups J, U and H. Non-parametric and multivariate analysis were performed to test the effects of the clinical variables, including gender, age, smoking status, diagnosis, mtDNA haplogroups and radiological Kellgren and Lawrence (K/L) grade on the serum levels of the molecular markers. RESULTS: Non-parametric analysis found increased serum levels of HA in patients with OA, while the values for C2C and the C2C/CPII ratio were significantly higher in the healthy controls. A multiple regression analysis showed a relationship between the mtDNA haplogroups and serum levels of the typical collagen type II markers. Carriers of the mtDNA haplogroup H had higher levels while carriers of the mtDNA haplogroup J showed lower levels. Statistically significant interactions between mtDNA haplogroups and diagnosis and between mtDNA haplogroups and radiological K/L grade in the serum levels of molecular markers were also found. CONCLUSION: A new role for mtDNA haplogroups emerges from this work. The results suggest that the mtDNA haplogroups interact significantly with the serum levels of OA-related molecular markers, suggesting the possibility of their use as a complementary assay with these molecular markers.
http://hdl.handle.net/2268/75196
10.1136/ard.2009.117416

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
ARD-2010-Haplogrupos-Biomarkers-Collagen.pdfPublisher postprint307.4 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.