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| Reference : Proteomic and genomic modulations induced by gamma-irradiation of human blood lymphocytes. |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/2268/75157 | |||
| Proteomic and genomic modulations induced by gamma-irradiation of human blood lymphocytes. | |
| English | |
Turtoi, Andrei  [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >] | |
| Sharan, Rajesh [North-Eastern Hill University, Shillong, Meghalaya, India > Department of Biochemistry > > >] | |
| Srivastava, Alok [Panjab University, Chandigarh, India > Chemistry Department > > >] | |
| Schneeweiss, Frank [Research Centre Jülich, Jülich, Germany > Laboratory of Radiation Biology, Department of Safety and Radiation Protection, > > >] | |
| Oct-2010 | |
| International Journal of Radiation Biology | |
| Taylor & Francis Ltd | |
| 86 | |
| 10 | |
| 888-904 | |
| International | |
| 0955-3002 | |
| 1362-3095 | |
| [en] PURPOSE: Quantitative evaluation of early response proteins (ERPRO) and early response genes (ERG) following γ-irradiation of human lymphocytes; identification of specific proteins and genes as candidate biomarkers for the development of a novel biodosimeter.
MATERIALS AND METHODS: Human peripheral blood lymphocytes were exposed to clinically relevant doses (1, 2 and 4 Gy) of γ-radiation ex-vivo. Analyses of protein and gene expression modulation were conducted 2 h post-irradiation. Global modulations were monitored using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and DNA microarray analyses of the samples originating from one human donor. On the proteome level, both phosphorylated and non-phosphorylated proteins were considered. Proteins and genes of specific interest were further targeted using Western blot (WB) and real-time quantitative polymerase chain reaction (RT-qPCR) techniques, employing samples from several human donors (n=3). RESULTS: A set of ERPRO and ERG showing significant alterations 2 h post-γ-irradiation have been identified in human lymphocytes. The most radiation responsive genes and proteins indicated alterations of cellular structure (ß-actin, talin-1 [TLN1], talin-2, zyxin-2), immune and defence reactions (major histocompatibility complex binding protein-2 [MBP2], interleukin-17E and interferon-γ), cell cycle control (cyclin-dependent kinase inhibitor-1A [CDKN1A], mouse double minute-2, annexin-A6 [ANXA6], growth arrest and DNA-damage-inducible protein-α [GADD45A], proliferating cell nuclear antigen [PCNA], dual specificity phosphatase-2 and 8 [DUSP8]) as well as detoxification processes (peroxin-1) and apoptosis (B-cell lymphoma-2 binding component-3 [BBC3]). SUMMARY: The estimations of protein concentration modulation of TLN1 and CDKN1A, phosphorylation status of ANXA6 (dose range 0-2 Gy) and MBP2 as well as the alterations in the level of gene expressions of BBC3, DUSP8, GADD45A and PCNA appears to be of potential value for future biodosimetric applications. | |
| http://hdl.handle.net/2268/75157 | |
| 10.3109/09553002.2010.486016 |
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