ORBi: Thi Thao Do - Precision study on capillary electrophoresis methods for metacycline.

Reference : Precision study on capillary electrophoresis methods for metacycline.


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Pharmacy, pharmacology & toxicology Physical, chemical, mathematical & earth Sciences : Chemistry
	To cite this reference:	http://hdl.handle.net/2268/7498

Title :	Precision study on capillary electrophoresis methods for metacycline.
Language :	English
Author, co-author :	Thi, Thao Do [> > > >]
	Pomponio, Romeo [> > > >]
	Gotti, Roberto [> > > >]
	Saevels, Jan [> > > >]
	Van Hove, Ben [> > > >]
	Van Ael, Willy [> > > >]
	Matthijs, Nele [> > > >]
	Vander Heyden, Yvan [> > > >]
	Marini Djang'Eing'A, Roland [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
	Chiap, Patrice [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
	Hubert, Philippe [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
	Crommen, Jacques [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Fabre, Huguette [> > > >]
	Dehouck, Pieter [> > > >]
	Hoogmartens, Jos [> > > >]
	Van Schepdael, Ann [> > > >]
Publication date :	2006
Journal title :	Electrophoresis
Publisher :	Vch Publishers
Volume :	27
Issue/season :	12
Pages :	2317-29
Audience :	International
ISSN :	0173-0835
City :	Weinheim
Country :	Germany
Keywords :	[en] Anti-Bacterial Agents/analysis ; Electrophoresis, Capillary/methods ; Methacycline/analysis ; Sensitivity and Specificity
Abstract :	[en] A CE method for metacycline (MTC) determination was investigated in an inter-laboratory experiment. Many problems were encountered in this study, most of which were related to the transfer of the method to different CE equipment. The reported problems could be classified into different categories: problems related to the precision, to the parameters in the protocol, and to the MTC peak shape. As the peak shape problem was partially responsible for the poor precision, a new CE method was developed in order to obtain a good MTC peak shape on all equipment. The precision of this new method for MTC determination was examined in an intermediate precision study, where the influence of the factors "time" and "equipment" was investigated. Although the new method could be transferred to different instruments, the precision remained poor mainly due to the contributions of the between-replicate and the between-injection variances.
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/7498
DOI :	10.1002/elps.200500873

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