Reference : Optimization of Matrix Conditions for the Control of MALDI In-Source Decay of Permethyla...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/74800
Optimization of Matrix Conditions for the Control of MALDI In-Source Decay of Permethylated Glycans.
English
Smargiasso, Nicolas mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
2010
Analytical Chemistry
American Chemical Society
sous presee
Yes (verified by ORBi)
International
0003-2700
1520-6882
Washington
DC
[en] Mass Spectrometry ; Glycans ; MALDI-ISD ; MALDI IMAGING
[en] Due to its fastness and its easiness to use, MALDI-MS is currently an analytical tool widely used in glycomic applications. However, the MALDI ionization process could result in the so-called "in-source decay", or ISD, of analytes, leading to complex spectra. On the other hand, ISD opens the possibility to perform pseudo-MS(3) experiments. This phenomenon must therefore be controlled in order to be used on demand as a supplementary tool for the analysis of permethylated glycans by MALDI mass spectrometry. For this purpose, several matrices were tested and MALDI imaging was used to determine optimal conditions promoting or, inversely, avoiding ISD of permethylated glycans. 2,5-DHB was shown to be a versatile matrix allowing one to induce or prevent ISD according to the location of laser shots. Inversely, it was shown that 9-aminoacridine forms homogeneous spots and avoids completely ISD. This matrix would therefore be suitable for automatic analysis.
Giga-Systems Biology and Chemical Biology ; Centre Interfacultaire d'Analyse des Résidus en Traces - CART
Fonds Européen de Développement Régional - FEDER ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
GLYCO (FEDER)
Researchers ; Professionals
http://hdl.handle.net/2268/74800
10.1021/ac1017452
http://pubs.acs.org/doi/abs/10.1021/ac1016198
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