Reference : New Advances in the Understanding of the In-Source Decay Fragmentation of Peptides in...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/74788
New Advances in the Understanding of the In-Source Decay Fragmentation of Peptides in MALDI-TOF-MS.
English
Demeure, Kevin [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Gabelica, Valérie mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
2010
Journal of the American Society for Mass Spectrometry
Elsevier Science
21
11
1906-17
Yes (verified by ORBi)
International
1044-0305
New York
NY
[en] Mass Spectrometry ; MALDI ; Peptides sequencing ; In source decay
[en] In-source decay (ISD) is a rapid fragmentation occurring in the matrix-assisted laser desorption/ionization (MALDI) source before the ion extraction. Despite the increasing interest for peptides de novo sequencing by ISD, the influence of the matrix and of the peptide itself is not yet fully understood. Here we compare matrices with high ISD efficiencies to gain deeper insight in the ISD fragmentation process(es). The major ISD fragments are the c- and z-ions, but other types of fragments are also observed, and their origin is studied here. Two main pathways lead to fragmentation in the source: a radical-induced pathway that leads to c-, z-, w-, and d-ions, and a thermally activated pathway that leads to y-, b-, and a-ions. A detailed analysis of the ISD spectra of selected peptides revealed that (1) the extents of the two in-source pathways are differently favored depending on the matrix used, that (2) the presence of a positive/negative charge on the radical-induced fragments is necessary for their observation in positive/negative mode, respectively, and that (3), for a same peptide, the patterns of the different types of fragments differ according to the matrix used.
Giga-Systems Biology and Chemical Biology ; Centre Interfacultaire d'Analyse des Résidus en Traces - CART
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Thèse de doctorat de Kevin Demeure
Researchers ; Professionals
http://hdl.handle.net/2268/74788
also: http://hdl.handle.net/2268/72938
10.1016/j.jasms.2010.07.009
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TH2-50NYWV3-1&_user=10&_coverDate=11%2F30%2F2010&_rdoc=14&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info%28%23toc%235270%232010%23999789988%232536737%23FLA%23display%23Volume%29&_cdi=5270&_sort=d&_docanchor=&_ct=20&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=3a15522bc796748f64fcb82e6932f999&searchtype=a
Copyright (c) 2010. Published by Elsevier Inc.
Copyright (c) 2010 American Society for Mass Spectrometry. Published by Elsevier Inc. All rights reserved.

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
JASMSNov2010KD.pdfPublisher postprint1.27 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.