Article (Scientific journals)
New Advances in the Understanding of the In-Source Decay Fragmentation of Peptides in MALDI-TOF-MS.
Demeure, Kevin; Gabelica, Valérie; De Pauw, Edwin
2010In Journal of the American Society for Mass Spectrometry, 21 (11), p. 1906-17
Peer Reviewed verified by ORBi
 

Files


Full Text
JASMSNov2010KD.pdf
Publisher postprint (1.3 MB)
Request a copy

Copyright © 2010. Published by Elsevier Inc.


All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Mass Spectrometry; MALDI; Peptides sequencing; In source decay
Abstract :
[en] In-source decay (ISD) is a rapid fragmentation occurring in the matrix-assisted laser desorption/ionization (MALDI) source before the ion extraction. Despite the increasing interest for peptides de novo sequencing by ISD, the influence of the matrix and of the peptide itself is not yet fully understood. Here we compare matrices with high ISD efficiencies to gain deeper insight in the ISD fragmentation process(es). The major ISD fragments are the c- and z-ions, but other types of fragments are also observed, and their origin is studied here. Two main pathways lead to fragmentation in the source: a radical-induced pathway that leads to c-, z-, w-, and d-ions, and a thermally activated pathway that leads to y-, b-, and a-ions. A detailed analysis of the ISD spectra of selected peptides revealed that (1) the extents of the two in-source pathways are differently favored depending on the matrix used, that (2) the presence of a positive/negative charge on the radical-induced fragments is necessary for their observation in positive/negative mode, respectively, and that (3), for a same peptide, the patterns of the different types of fragments differ according to the matrix used.
Research center :
Giga-Systems Biology and Chemical Biology - ULiège
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Chemistry
Author, co-author :
Demeure, Kevin ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Gabelica, Valérie ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Language :
English
Title :
New Advances in the Understanding of the In-Source Decay Fragmentation of Peptides in MALDI-TOF-MS.
Publication date :
2010
Journal title :
Journal of the American Society for Mass Spectrometry
ISSN :
1044-0305
eISSN :
1879-1123
Publisher :
Elsevier Science, New York, United States - New York
Volume :
21
Issue :
11
Pages :
1906-17
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Thèse de doctorat de Kevin Demeure
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Commentary :
Copyright (c) 2010 American Society for Mass Spectrometry. Published by Elsevier Inc. All rights reserved.
Available on ORBi :
since 01 November 2010

Statistics


Number of views
58 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
81
Scopus citations®
without self-citations
66
OpenCitations
 
78

Bibliography


Similar publications



Contact ORBi