Reference : Sera from patients with anti-GBM nephritis including goodpasture syndrome show heterogen...
Scientific journals : Article
Human health sciences : Urology & nephrology
Sera from patients with anti-GBM nephritis including goodpasture syndrome show heterogenous reactivity to recombinant NC1 domain of type IV collagen alpha chains.
Dehan, Pierre mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Weber, M. [> >]
Zhang, X. [> >]
Reeders, S. T. [> > > >]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]
Tryggvason, K. [> > > >]
Nephrology Dialysis Transplantation
Oxford University Press
Yes (verified by ORBi)
United Kingdom
[en] Adult ; Aged ; Anti-Glomerular Basement Membrane Disease/blood/immunology ; Autoantibodies/blood ; Biological Markers ; Collagen/genetics/immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoblotting ; Male ; Middle Aged ; Recombinant Proteins/immunology
[en] BACKGROUND: Goodpasture (GP) syndrome is defined by the clinical association of pulmonary haemorrhage with rapidly progressive glomerulonephritis. The disease is caused by pathogenic autoantibodies directed against type IV collagen, which is a major structural component of glomerular basement membranes (GBM). METHODS: The non-collagenous domains (NC1) of all six human type IV collagen alpha chains was produced in E. coli as recombinant fusion proteins with glutathione-S transferase. Sera from 10 patients with different types of anti-GBM nephritis, including GP syndrome, were tested for reactivity with the six proteins using immunoblotting of denatured and reduced proteins and ELISA without reduction. RESULTS: All 10 sera reacted with the alpha 3 (IV) collagen chain by immunoblotting and ELISA. One serum also recognized the alpha 2(IV), alpha 4(IV), alpha 5(IV) and alpha 6(IV) chains by immunoblotting. ELISA measurements revealed reactivity of several other sera with alpha 2(IV), alpha 4(IV) or alpha 6(IV) but not with alpha 5(IV) collagen chains. No reactivity was observed with the alpha 1(IV) chain. CONCLUSION: Autoantibodies in anti-GBM nephritis may not be directed only against the alpha 3(IV) collagen chain and they frequently recognize conformational epitopes.

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