Article (Scientific journals)
A reduced extracellular serotonin level increases the 5-HT1A PET ligand F-18-MPPF binding in the rat hippocampus
Zimmer, L.; Rbah, L.; Giacomelli, Fabrice et al.
2003In Journal of Nuclear Medicine, 44 (9), p. 1495-1501
Peer Reviewed verified by ORBi
 

Files


Full Text
JNuclMed_2003_1495.pdf
Publisher postprint (151.59 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
serotonin receptors; p-ethynylphenylalanine; 4,2'(methoxyphenyl)-1-[2'-(N-2"-pyridinyl)-p-fluorobenzamido]-ethylpiperazine; serotonin; microdialysis; beta-microprobe
Abstract :
[en] 4,2'-(Methoxyphenyl)-1-[2'-(N-2"-pyridinyl)-p-fluorobenzamido]ethylpiperazine (F-18-MPPF) is a radiotracer used in clinical PET studies for the visualization of serotonin-1A (5-HT1A) receptors. In a previous study, we demonstrated that a rapid enhancement of extracellular serotonin concentrations influences F-18-MPPF-specific binding. Because endogenous serotonin is significantly decreased in some pathologies, the aim of this study was to determine whether F-18-MPPF is sensitive to depletion of this neurotransmitter. Methods: Using the beta-microprobe, an original beta(+)-sensitive intracerebral probe, and micro-dialysis, the effect of decreased serotonin on the specific binding of F-18-MPPF to 5-HT1A receptors was investigated in the hippocampus of the anesthetized rat. Extracellular serotonin was pharmacologically decreased in the hippocampus after a single injection of p-ethynylphenylalanine ([p-EPA] 5 mg/kg), a new tryptophan hydroxylase inhibitor. Results: Our results showed that the F-18-MPPF-specific binding was significantly enhanced after the decrease of extracellular serotonin. These results were confirmed by the F-18-MPPF distribution in cerebral tissues (hippocampus-to-cerebellum ratio) and by the decrease of the extracellular F-18-MPPF collected in hippocampal dialysates. Conclusion: This study further supports the view that 18F-MPPF binding potential is increased in the hippocampus if the endogenous serotonin is pharmacologically decreased after a p-EPA injection. This phenomenon will be an additional factor in the interpretation of the results from F-18-MPPF clinical PET studies.
Research center :
GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Zimmer, L.;  CERMEP
Rbah, L.
Giacomelli, Fabrice ;  Université de Liège - ULiège > Centre de recherches du cyclotron
Le Bars, D.;  CERMEP
Renaud, B.
Language :
English
Title :
A reduced extracellular serotonin level increases the 5-HT1A PET ligand F-18-MPPF binding in the rat hippocampus
Publication date :
September 2003
Journal title :
Journal of Nuclear Medicine
ISSN :
0161-5505
eISSN :
1535-5667
Publisher :
Society of Nuclear Medicine, Reston, United States - Virginia
Volume :
44
Issue :
9
Pages :
1495-1501
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 October 2010

Statistics


Number of views
23 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
41
Scopus citations®
without self-citations
32

Bibliography


Similar publications



Contact ORBi