Article (Scientific journals)
Fully Automated Determination of Sulfamethazine in Ovine Plasma Using Solid-Phase Extraction on Disposable Cartridges and Liquid Chromatography
Hubert, Philippe; Chiap, Patrice; Evrard, Brigitte et al.
1993In Journal of Chromatography. A, 622 (1), p. 53-60
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Abstract :
[en] An automatic sample preparation procedure followed by on-line injection of the sample extract into a HPLC system has been developed for the quantitative analysis of sulfamethazine and its N4-acetyl metabolite in ovine plasma. The sample clean-up was performed by solid-phase extraction (SPE) on C18 disposable extraction cartridges (DECs). All the sample handling operations were effected by a robotic auto-sampler. The DEC was first conditioned with methanol and phosphate buffer pH 7.4. After loading 1.0 ml of plasma sample onto the DEC, the latter was washed with the same buffer. The elution step was performed with methanol (0.25 ml) and the eluate was then diluted by adding 0.75 ml volume of phosphate buffer pH 6.4. A 20-microliters volume of the resultant solution was injected onto an octadecyl silica column preceded by a short guard column. The HPLC mobile phase was methanol-phosphate buffer pH 6.4 (25:75, v/v). Sulfamethazine and N4-acetylsulfamethazine were determined photometrically at 262 nm. Under these conditions, linear calibration curves ranging from 2 to 250 micrograms ml-1 have been obtained for both compounds. Drug recoveries were higher than 90% and typical relative standard deviation values were 0.7% (within-day) and 2.0% (between-day) at a plasma concentration of 50 micrograms ml-1.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Hubert, Philippe  ;  Université de Liège - ULiège > Chimie analytique
Chiap, Patrice ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Evrard, Brigitte  ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Delattre, Luc ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Crommen, Jacques ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Fully Automated Determination of Sulfamethazine in Ovine Plasma Using Solid-Phase Extraction on Disposable Cartridges and Liquid Chromatography
Publication date :
08 December 1993
Journal title :
Journal of Chromatography. A
ISSN :
0021-9673
eISSN :
1873-3778
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
622
Issue :
1
Pages :
53-60
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 March 2009

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