Reference : Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic n...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/73952
Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation
English
Vlieghe, Kobe [Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology > >]
Vuylsteke, Marnik [Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology > >]
Florquin, Kobe [Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology > >]
Rombauts, Stéphane [Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology > >]
Maes, Sara [Ghent University > Flanders Interuniversity Institute for Biotechnology (VIB) > Department of Plant Systems Biology > >]
Ormenese, Sandra mailto [Université de Liège - ULg > > GIGA-Management : Plate-forme imagerie >]
Van Hummelen, Paul [> > > >]
Van de Peer, Yves [> > > >]
Inze, Dirk [> > > >]
De Veylder, Lieven [> > > >]
15-Oct-2003
Journal of Cell Science
Company Of Biologists Ltd
116
20
4249-4259
Yes (verified by ORBi)
0021-9533
Cambridge
[en] Arabidopsis thaliana ; cell cycle ; E2F ; microarray ; nitrogen assimilation
[en] dPreviously we have shown that overexpression of the heterodimeric E2Fa-DPa transcription factor in Arabidopsis thaliana results in ectopic cell division, increased endoreduplication, and an early arrest in development. To gain a better insight into the phenotypic behavior of E2Fa-DPa transgenic plants and to identify E2Fa-DPa target genes, a transcriptomic microarray analysis was performed. Out of 4,390 unique genes, a total of 188 had a twofold or more up- (84) or down-regulated (104) expression level in E2Fa-DPa transgenic plants compared to wild-type lines. Detailed promoter analysis allowed the identification of novel E2Fa-DPa target genes, mainly involved in DNA replication. Secondarily induced genes encoded proteins involved in cell wall biosynthesis, transcription and signal transduction or had an unknown function. A large number of metabolic genes were modified as well, among which, surprisingly, many genes were involved in nitrate assimilation. Our data suggest that the growth arrest observed upon E2Fa-DPa overexpression results at least partly from a nitrogen drain to the nucleotide synthesis pathway, causing decreased synthesis of other nitrogen compounds, such as amino acids and storage proteins.
http://hdl.handle.net/2268/73952

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