Reference : Three-dimensional electron microscopy of ribosomal chromatin in two higher plants: a ...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Three-dimensional electron microscopy of ribosomal chromatin in two higher plants: a cytochemical, immunocytochemical, and in situ hybridization approach.
Motte, Patrick mailto [Université de Liège - ULg > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale >]
Loppes, Roland mailto [Université de Liège - ULg > > Génétique des microorganismes >]
Menager, M. [> > > >]
Deltour, Roger mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Histochemical Society
Yes (verified by ORBi)
New York
[en] Chromatin/ultrastructure ; DNA/metabolism ; Immunohistochemistry ; Karyotyping ; Microscopy, Electron ; Nucleic Acid Conformation ; Plants/metabolism ; Ribosomes/metabolism/ultrastructure
[en] We report the 3-D arrangement of DNA within the nucleolar subcomponents from two evolutionary distant higher plants, Zea mays and Sinapis alba. These species are particularly convenient to study the spatial organization of plant intranucleolar DNA, since their nucleoli have been previously reconstructed in 3-D from serial ultra-thin sections. We used the osmium ammine-B complex (a specific DNA stain) on thick sections of Lowicryl-embedded root fragments. Immunocytochemical techniques using anti-DNA antibodies and rDNA/rDNA in situ hybridization were also applied on ultra-thin sections. We showed on tilted images that the OA-B stains DNA throughout the whole thickness of the section. In addition, very low quantities of cytoplasmic DNA were stained by this complex, which is now the best DNA stain used in electron microscopy. Within the nucleoli the DNA was localized in the fibrillar centers, where large clumps of dense chromatin were also visible. In the two plant species intranucleolar chromatin forms a complex network with strands partially linked to chromosomal nucleolar-organizing regions identified by in situ hybridization. This study describes for the first time the spatial arrangement of the intranucleolar chromatin in nucleoli of higher plants using high-resolution techniques.
Researchers ; Professionals

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