Article (Scientific journals)
Resveratrol and curcumin reduce the respiratory burst of Chlamydia-primed THP-1 cells
Deby-Dupont, Ginette; Mouithys-Mickalad, Ange; Serteyn, Didier et al.
2005In Biochemical and Biophysical Research Communications, 333 (1), p. 21-27
Peer reviewed
 

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Keywords :
THP-1; Chlamydia pneumoniae; resveratrol; curcumin; NADPH oxidase; priming; IL-8; TNF alpha
Abstract :
[en] The intracellular bacterium Chlamydia pneumoniae is involved in the inflammation process of atherosclerosis. We previously demonstrated that C. pneumonia infected monocytes (THP-1 cells) responded to stimulation by an increased respiratory burst linked to an increased NADPH oxidase (NOX) activity. We now tested agents acting on the assembly of the NOX subunits or on protein kinase C, a trigger of NOX activity. Apocynin, resveratrol, rutin, quercetin, curcumin, and tocopherols were tested. The cells were pre-incubated with Chlamydia and the agent for 19 h, and then stimulated with phorbol myristate acetate. The NOX activity was monitored by measuring the hydrogen peroxide production. Resveratrol and curcumin (10(-4)-10(-6) M) were better inhibitors than apocynin. alpha-Tocopherol was inactive, and gamma-tocopherol inhibitor at 10(-4) M only. Quercetin was inactive, and rutin a moderate but significant inhibitor. The inhibition by resveratrol was increased by 10(-6) M rutin or quercetin. Resveratrol and curcumin thus appeared to be interesting for atherosclerosis treatment.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Deby-Dupont, Ginette
Mouithys-Mickalad, Ange ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Lamy, Maurice ;  Université de Liège - ULiège > Département des sciences cliniques > Anesthésie et réanimation
Deby, Carol
Language :
English
Title :
Resveratrol and curcumin reduce the respiratory burst of Chlamydia-primed THP-1 cells
Publication date :
22 July 2005
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press, San Diego, United States - California
Volume :
333
Issue :
1
Pages :
21-27
Peer reviewed :
Peer reviewed
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