Reference : ADAMTS-2 functions as anti-angiogenic and anti-tumoral molecule independently of its ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/73235
ADAMTS-2 functions as anti-angiogenic and anti-tumoral molecule independently of its catalytic activity.
English
Dubail, Johanne [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Kesteloot, F. [> > > >]
Deroanne, Christophe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Motte, Patrick mailto [Université de Liège - ULg > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale >]
Lambert, Vincent mailto [Centre Hospitalier Universitaire de Liège - CHU > > Ophtalmologie >]
Rakic, Jean-Marie mailto [Centre Hospitalier Universitaire de Liège - CHU > > Ophtalmologie >]
Lapiere, C. [> > > >]
Nusgens, Betty mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Colige, Alain mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
2010
Cellular & Molecular Life Sciences
Springer Science & Business Media B.V.
Yes (verified by ORBi)
International
1420-682X
1420-9071
Basel
Switzerland
[en] ADAMTS ; Angiogenesis ; Cell adhesion ; Apoptosis ; Tumor
[en] ADAMTS-2 is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. We demonstrate that recombinant ADAMTS-2 is also able to reduce proliferation of endothelial cells, and to induce their retraction and detachment from the substrate resulting in apoptosis. Dephosphorylation of Erk1/2 and MLC largely precedes the ADAMTS-2 induced morphological alterations. In 3-D culture models, ADAMTS-2 strongly reduced branching of capillary-like structures formed by endothelial cells and their long-term maintenance and inhibited vessels formation in embryoid bodies (EB). Growth and vascularization of tumors formed in nude mice by HEK 293-EBNA cells expressing ADAMTS-2 were drastically reduced. A similar anti-tumoral activity was observed when using cells expressing recombinant deleted forms of ADAMTS-2, including catalytically inactive enzyme. Nucleolin, a nuclear protein also found to be associated with the cell membrane, was identified as a potential receptor mediating the antiangiogenic properties of ADAMTS-2.
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/73235
10.1007/s00018-010-0431-6

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