Reference : High frequency of RASSF1A and RARb2 gene promoter methylation in morphologically normal ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/7321
High frequency of RASSF1A and RARb2 gene promoter methylation in morphologically normal endometrium adjacent to endometrioid adenocarinoma
English
Arafa, M. [> >]
Kridelka, Frédéric mailto [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique >]
Mathias, Valérie mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Vanbellinghen, Jean-François [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Renard, I. [> >]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]
Boniver, Jacques mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Delvenne, Philippe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
2008
Histopathology
Blackwell Publishing
53
5
525-532
Yes (verified by ORBi)
International
0309-0167
1365-2559
Oxford
United Kingdom
[en] carcinoma ; DNA methylation ; endometrium ; RARb2 ; RASSF1A
[en] Aims: To identify a DNA methylation signature of endometrioid carcinoma of the endometrium (EEC) in the early stages of endometrial carcinogenesis.
<br />Methods and results: Archival biopsy specimens of 39 EECs, 14 cases of atypical hyperplasia (AH), 11 histologically normal endometrial tissues adjacent to EECs and 24 normal control endometrial samples were retrieved. The cases were tested by quantitative methylation-specific polymerase chain reaction with primers hybridizing in the promoter regions of five genes frequently methylated in human cancer (RASSF1A, RARb2, P16, MGMT and GSTPi). Twenty-nine of 39 (74%) EECs and 7/14 (50%) AHs were methylated for the RASSF1A gene, whereas 17/39 (44%) EECs and 6/14 (43%) AHs were positive for the methylation of the RARb2 gene. No significant results were obtained for the other genes (P16, MGMT and GSTPi). Interestingly, 4/11 (36%) and 6/11 (55%) histologically normal endometrial tissues adjacent to EEC showed, respectively, RASSF1A and RARb2 gene methylation. Furthermore, these 11 specimens were microsatellite stable and showed similar proliferative, cell cycle and apoptotic mean labelling indices as the normal endometrial control tissues.
<br />Conclusions: Promoter region methylation of RASSF1A and RARb2 genes is an early event in endometrial carcinogenesis.
http://hdl.handle.net/2268/7321
also: http://hdl.handle.net/2268/8154
10.1111/j.1365-2559.2008.03147.x

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