Reference : A simple method for the preparation of covalently closed circular form of plasmid DNA

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/73195

Title :	A simple method for the preparation of covalently closed circular form of plasmid DNA
Language :	English
Author, co-author :	Le Brun, J. J. [Université d'Angers, France > INSERM U 298 > > >]
	Rentier-Delrue, Françoise [Université de Liège - ULg > > Laboratoire Central de Génie génétique > >]
	Mercier, L. [Université d'Angers, France > > INSERM U 298 > >]
Publication date :	1988
Journal title :	BioTechniques
Publisher :	Eaton Publishing Company
Audience :	International
ISSN :	0736-6205
City :	Natick
Country :	MA
Keywords :	[en] Plasmid DNA ; Covalently closed circular form ; purification
Abstract :	[en] We describe a simple, rapid, inexpensive method for isolation of covalently closed circular plasmid DNA. The method involves the electrophoresis of crude DNA preparations in an agarose gel, electrotransfer onto a dialysis membrane and elution of the highly purified circular covalently closed plasmid DNA. Native and recombinant plasmid DNA have been purified by this method and shown to be suitable for restriction enzyme digestion and transformation of bacteria. The yield of this rapid purification procedure makes it a good alternative method to standard centrifugation in cesium chloride ethidium bromide gradients.
Permalink :	http://hdl.handle.net/2268/73195

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