Detection of mRNA encoding an antibacterial-metalloprotein (MPII) by in situ hybridization with a cDNA probe generated by PCR in the worm nereis diversicolor
Salzet-Raveillon, B.; Rentier-Delrue, Françoise; Dhainaut, A.
1993 • In Cellular and Molecular Biology, p. 105-114
antibacterial-metalloprotein; in situ hybridization
Abstract :
[en] Based on partial amino acid sequence of an antibacterial-metalloprotein (MPII) consisting of a N-terminal fragment (1-33th) and an 43 amino acids long internal fragment, two oligonucleotides primers were synthesized and used to generate a cDNA fragment by the PCR method. The specificity of the PCR synthesized 220 bp fragment was verified by hybrid-arrest translation and sequencing. In situ hybridization performed with this cDNA fragment on Nereis diversicolor whole body defined two specific sites of recognition: a cluster of cells floating in the coelom and two types of muscles (perineural and oblique). Finally, the chronological expression of the MPII was postulated.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Salzet-Raveillon, B.; Université des Sciences et Technologies de Lille - USTL > CNRS 20
Dhainaut, A.; Université des Sciences et Technologies de Lille - USTL > CNRS 20
Language :
English
Title :
Detection of mRNA encoding an antibacterial-metalloprotein (MPII) by in situ hybridization with a cDNA probe generated by PCR in the worm nereis diversicolor
Publication date :
1993
Journal title :
Cellular and Molecular Biology
ISSN :
0145-5680
eISSN :
1165-158X
Publisher :
Hospital Publications, Secaucus, United States - New Jersey