|Reference : Clostridium perfringens urease genes are plasmid-borne.|
|Scientific journals : Article|
|Life sciences : Food science|
|Clostridium perfringens urease genes are plasmid-borne.|
|Dupuy, B. [ > > ]|
|Daube, Georges [Université de Liège - ULg > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires >]|
|Popoff, M. R. [ > > ]|
|Cole, S. T. [ > > ]|
|Infection and Immunity|
|American Society for Microbiology (ASM)|
|[en] Clostridium perfringens ; Urease gene|
|[en] Although many bacteria are ureolytic, and in some cases urease acts as a virulence factor, the urease
phenotype has not been analyzed in the anaerobic pathogen Clostridium perfringens. In this study, ;2% of C.
perfringens strains, representing the principal biotypes, were found to harbor the urease structural genes,
ureABC, and these were localized on large plasmids that often encode, in addition, the lethal « or i toxins or
the enterotoxin. This represents the first report of a plasmid-encoded urease in a gram-positive bacterium. The
C. perfringens enzyme was highly similar to the ureases of other bacteria and cross-reacted with antibodies
raised against the urease purified from Helicobacter pylori. Urease production was inhibited by urea and
induced under growth conditions where the availability of nitrogen sources was limiting. To date, this form of
regulation has been observed only for chromosomal ureABC genes.
|Researchers ; Students|
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