ORBi: Horsnell W. G. - IL-4Ralpha-responsive smooth muscle cells contribute to initiation of T(H)2 immunity and pulmonary pathology in Nippostrongylus brasiliensis infections.

Reference : IL-4Ralpha-responsive smooth muscle cells contribute to initiation of T(H)2 immunity and...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Immunology & infectious disease
	To cite this reference:	http://hdl.handle.net/2268/72478

Title :	IL-4Ralpha-responsive smooth muscle cells contribute to initiation of T(H)2 immunity and pulmonary pathology in Nippostrongylus brasiliensis infections.
Language :	English
Author, co-author :	Horsnell, W. G. [> > > >]
	Vira, A. [> > > >]
	Kirstein, F. [> > > >]
	Mearns, H. [> > > >]
	Hoving, J. C. [> > > >]
	Cutler, A. J. [> > > >]
	Dewals, Benjamin G [Université de Liège - ULg > > Immunologie et vaccinologie >]
	Myburgh, E. [> > > >]
	Kimberg, M. [> > > >]
	Arendse, B. [> > > >]
	White, N. [> > > >]
	Lopata, A. [> > > >]
	Burger, P. E. [> > > >]
	Brombacher, F. [> > > >]
Publication date :	Jan-2010
Journal title :	Mucosal immunology
Volume :	4
Issue/season :	1
Pages :	83-92
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	1933-0219
e-ISSN :	1935-3456
Abstract :	[en] Nippostrongylus brasiliensis infections generate pulmonary pathologies that can be associated with strong T(H)2 polarization of the host's immune response. We present data demonstrating N. brasiliensis-driven airway mucus production to be dependent on smooth muscle cell interleukin 4 receptor-alpha (IL-4Ralpha) responsiveness. At days 7 and 10 post infection (PI), significant airway mucus production was found in IL-4Ralpha(-/lox) control mice, whereas global knockout (IL-4Ralpha(-/-)) and smooth muscle-specific IL-4Ralpha-deficient mice (SM-MHC(Cre) IL-4Ralpha(-/lox)) showed reduced airway mucus responses. Furthermore, interleukin (IL)-13 and IL-5 cytokine production in SM-MHC(Cre) IL-4Ralpha(-/lox) mice was impaired along with a transient reduction in T-cell numbers in the lung. In vitro treatment of smooth muscle cells with secreted N. brasiliensis excretory-secretory antigen (NES) induced IL-6 production. Decreased protein kinase C (PKC)-dependent smooth muscle cell proliferation associated with cell cycle arrest was found in cells stimulated with NES. Together, these data demonstrate that both IL-4Ralpha and NES-driven responses by smooth muscle cells make important contributions in initiating T(H)2 responses against N. brasiliensis infections.Mucosal Immunology advance online publication 25 August 2010. doi:10.1038/mi.2010.46.
Permalink :	http://hdl.handle.net/2268/72478
DOI :	10.1038/mi.2010.46

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