Reference : Image analysis of the axonal ingrowth into poly(D,L-lactide) porous scaffolds in relatio...
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
Physical, chemical, mathematical & earth Sciences : Chemistry
Engineering, computing & technology : Materials science & engineering
http://hdl.handle.net/2268/7233
Image analysis of the axonal ingrowth into poly(D,L-lactide) porous scaffolds in relation to the 3-D porous structure
English
Blacher, Silvia mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Maquet, Véronique [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Luyckx, Françoise mailto [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
Martin, Didier mailto [Université de Liège - ULg > Département des sciences cliniques > Neurochirurgie]
Schoenen, Jean mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Neuro-anatomie]
Moonen, Gustave mailto [Université de Liège - ULg > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine]
Jérôme, Robert mailto [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Pirard, Jean-Paul mailto [Université de Liège - ULg > Département de chimie appliquée > Génie chimique - Génie catalytique >]
Mar-2003
Biomaterials
Elsevier
24
6
1033-1040
Yes (verified by ORBi)
International
0142-9612
Oxford
[en] Porous polymer scaffolds ; Axonal growth ; Image analysis ; Anisotropy ; biomaterial ; scaffold
[en] Porous polymer scaffolds are promising materials for neural tissue engineering because they offer valuable three-dimensional (3D) supports for the in vitro and in vivo axonal growth and tissue expansion. At the time being, how the in vivo neuronal cell development depends on the scaffold 3-D architecture is unknown. Therefore, scanning electron micrographs of longitudinal sections of porous polylactide scaffolds and immunohistological sections of these scaffolds after implantation and neurofilament staining have been studied by image analysis. Pore orientation and axonal ingrowth have been investigated by spectral analysis on gray level SEM images. Binary image processing has been carried out and the binary images have been studied by spectral analysis in order to estimate the possible effect of the image noise on the real pattern. In addition to axonal orientation, density and length distribution of the regenerated axons into the polymer scaffold have been measured. Dependence of the axonal ingrowth on the 3D-polymer scaffold has been discussed on the basis of the collected data. (C) 2002 Elsevier Science Ltd. All rights reserved.
Center for Education and Research on Macromolecules (CERM)
Politique Scientifique Fédérale (Belgique) = Belgian Federal Science Policy ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers
http://hdl.handle.net/2268/7233
10.1016/S0142-9612(02)00423-4
http://www.elsevier.com/wps/find/journaldescription.cws_home/30392/description#description
http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TWB-47GFNJM-K-17&_cdi=5558&_user=532038&_orig=browse&_coverDate=03%2F31%2F2003&_sk=999759993&view=c&wchp=dGLzVtz-zSkWz&md5=960b47f1b5305d33ad59999b6ee3224b&ie=/sdarticle.pdf
The authors acknowledge Biomaterials (Elsevier) for allowing them to archive this paper.

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