Reference : Evi1 is specifically expressed in the distal tubule and duct of the Xenopus pronephro...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/72121
Evi1 is specifically expressed in the distal tubule and duct of the Xenopus pronephros and plays a role in its formation.
English
Van Campenhout, Claude [Université Libre de Bruxelles - ULB > Institut de Biologie et de Médecine Moléculaires > Laboratoire d'Embryologie Moléculaire > >]
Nichane, Massimo [Université Libre de Bruxelles - ULB > Institut de Biologie et de Médecine Moléculaires > Laboratoire d'Embryologie Moléculaire > >]
Antoniou, Aline [Université Libre de Bruxelles - ULB > Institut de Biologie et de Médecine Moléculaires > Laboratoire d'Embryologie Moléculaire > >]
Pendeville, Helene [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire > >]
Bronchain, Odile J [Université Paris-Sud 11 > CNRS UMR 8080, IBAIC, > Transgenèse et Génétique des Amphibiens > >]
Marine, Jean-Christophe [University of Ghent > Flanders Interuniversity Institute for Biotechnology > Laboratory for Molecular Cancer Biology > >]
Mazabraud, Andre [Université Paris-Sud 11 > CNRS UMR 8080, IBAIC > Transgenèse et Génétique des Amphibiens > >]
Voz, Marianne mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire > >]
Bellefroid, Eric J. [Université Libre de Bruxelles - ULB > Institut de Biologie et de Médecine Moléculaires > Laboratoire d'Embryologie Moléculaire > >]
2006
Developmental Biology
Academic Press
294
1
203-19
Yes (verified by ORBi)
International
0012-1606
1095-564X
Orlando
FL
[en] Amino Acid Sequence ; Animals ; Carrier Proteins ; Gene Expression Regulation, Developmental ; Kidney/embryology/growth & development ; Membrane Proteins ; Morphogenesis ; Sequence Alignment ; Thyroid Hormones ; Transcription Factors/genetics/physiology ; Transcription, Genetic ; Up-Regulation ; Xenopus Proteins/genetics ; Xenopus laevis
[en] The ecotropic viral integration site 1 (Evi1) and related MEL1 (MDS1/Evi1-like gene 1) genes are zinc finger oncogenic transcription factors involved in myeloid leukaemia. Here, we show that in Xenopus, Evi1 and MEL1 have partially overlapping restricted embryonic expression profiles. Within the pronephros, Evi1 and MEL1 are sequentially expressed within the distal tubule and duct compartments, Evi1 transcription being detected prior to any sign of pronephric morphogenesis. In the pronephros of zebrafish embryos, Evi1 expression is restricted to the posterior portion of the duct, the anterior portion having characteristics of proximal tubules. In the Xenopus pronephros, Evi1 expression is upregulated by retinoid signaling and repressed by overexpression of xWT1 and by Notch signaling. Overexpression of Evi1 from late neurula stage specifically inhibits the expression of proximal tubule and glomus pronephric markers. We show that the first zinc finger and CtBP interaction domains are required for this activity. Overexpression of a hormone-inducible Evi1-VP16 antimorphic fusion with activation at neurula stage disrupts distal tubule and duct formation and expands the expression of glomus markers. Although overexpression of this construct also causes in many embryos a reduction of proximal tubule markers, embryos with expanded and ectopic staining have been also observed. Together, these data indicate that Evi1 plays a role in the proximo-distal patterning of the pronephros and suggest that it may do so by functioning as a CtBP dependent repressor.
Giga-Development and Stem Cells
Researchers ; Professionals
http://hdl.handle.net/2268/72121
10.1016/j.ydbio.2006.02.040
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WDG-4JKRTW7-4&_user=532038&_coverDate=06%2F01%2F2006&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=9ee90cd12939ccebe6e92fe69d3cfc23&searchtype=a

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
Evi1 OCR.docAuthor preprint18.72 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.