Reference : In Vitro and in Vivo Modulation of 5-Hydroxytryptamine-, Thyrotropin-Releasing Hormone- ...
Scientific journals : Article
Human health sciences : Surgery
http://hdl.handle.net/2268/7206
In Vitro and in Vivo Modulation of 5-Hydroxytryptamine-, Thyrotropin-Releasing Hormone- and Calcitonin-Gene Related Peptide-Like Immunoreactivities in Adult Rat Sensory Neurons
English
Delree, P. [> > > >]
Martin, Didier mailto [Université de Liège - ULg > Département des sciences cliniques > Neurochirurgie]
Sadzot-Delvaux, Catherine mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie et immunologie - GIGA-M : Coordination scientifique >]
Rogister, Bernard mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
Leprince, Pierre mailto [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Robe, Pierre mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Génétique générale et humaine >]
Rigo, Jean-Marie mailto [Université de Liège - ULg > Département Argenco : Secteur GeMMe > Matériaux non métal. & Procédés d'exécution des construct. >]
Lefebvre, P. P. [> > > >]
Malgrange, Brigitte mailto [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Schoenen, Jean mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Neuro-anatomie]
et al. [> > > >]
Nov-1992
Neuroscience
51
2
401-10
Yes (verified by ORBi)
International
0306-4522
[en] In a previous work we have shown that culturing adult rat dorsal root ganglia neurons modifies their neurotransmitter phenotype in such a way that cultured neurons synthesize transmitters that are not found in situ, while several other transmitters are expressed in a much higher percentage of neurons in culture than in situ [Schoenen J. et al. (1989) J. Neurosci. Res. 22, 473-487]. The aim of the present study was to investigate the origin and the nature of the relevant environmental signals that allow this plasticity to be expressed, focusing on three neurotransmitters: 5-hydroxytryptamine, thyrotropin-releasing hormone and calcitonin-gene related peptide. The main results can be summarized as follows: (1) culturing cells in fetal calf serum or on feeder layers of astrocytes, Schwann cells or fibroblasts partially inhibits the serotoninergic phenotype of dorsal root ganglia neurons; (2) in vivo disconnection of dorsal root ganglia from their spinal targets but not from their peripheral or supraspinal targets induces a significant increase of the percentage of 5-hydroxytryptamine- and thyrotropin-releasing hormone-positive neurons in disconnected ganglia; (3) growth factors such as ciliary neuronotrophic factor or basic fibroblast growth factor but not nerve growth factor repress 5-hydroxytryptamine and calcitonin gene-related peptide immunoreactivity in cultured sensory neurons. In conclusion, neurotransmitter gene expression of adult dorsal root ganglia neurons is controlled by complex influences. Our data suggest that thyrotropin-releasing hormone and 5-hydroxytryptamine gene expression are tonically repressed in vivo by factors originating from the spinal segmental level and that growth factors such as ciliary neurotrophic factor or basic fibroblast growth factor could be potential vectors of this repressing effect.
http://hdl.handle.net/2268/7206
also: http://hdl.handle.net/2268/7201

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Neuroscience 51 (1992) 401-410 - Delree - DRG phenotype.pdfNo commentaryPublisher postprint1.78 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.