Reference : Alpha-MSH stimulates neurite outgrowth of neonatal rat corticospinal neurons in vitro.
Scientific journals : Article
Human health sciences : Surgery
http://hdl.handle.net/2268/7202
Alpha-MSH stimulates neurite outgrowth of neonatal rat corticospinal neurons in vitro.
English
Joosten, E. A. [> > > >]
Verhaagh, S. [> > > >]
Martin, Didier mailto [Centre Hospitalier Universitaire de Liège - CHU > > Neurochirurgie >]
Robe, Pierre mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Génétique générale et humaine - Département des sciences biomédicales et précliniques >]
Franzen, Rachelle mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Neuro-anatomie >]
Hooiveld, M. [> > > >]
Doornbos, R. [> > > >]
Bartsch, Pierre mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Moonen, Gustave mailto [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
1996
Brain Research
Elsevier Science
736
1-2
91-8
Yes (verified by ORBi)
International
0006-8993
Amsterdam
The Netherlands
[en] Animals ; Animals, Newborn ; Axons/ultrastructure ; Cells, Cultured ; Cerebral Cortex/cytology ; Cholera Toxin ; Dendrites/ultrastructure ; Neurites/drug effects/physiology ; Neurons/drug effects/physiology/ultrastructure ; Rats ; Rats, Inbred Strains ; alpha-MSH/pharmacology
[en] Peptides related to melanocortin (alpha MSH) and corticotropin (ACTH), collectively termed melanocortins, exert trophic effects on the outgrowth of neurites from peripheral and central nervous system in vitro. Here we study the neurite outgrowth promoting effect of alpha-MSH on corticospinal (CS) neurons in vitro. Corticospinal neurons were identified in cell culture of neonatal rat cortex by immunostaining of cholera toxin subunit B (CTB), retrogradely transported from the cervical parts of the spinal cord. The CTB-immunoreactive neurons represent a small percentage (3-5%) of the total cell population after 72 h in vitro. The axons or dendrites of cortical and CTB-labelled layer V neurons were visualized using antibodies against axon- or dendrite-specific markers and measured using a semi-automatic quantification device. Here we report that alpha-MSH stimulates axonal as well as dendrite outgrowth from both total and CTB-labelled neurons with a bell-shape response curve. Axonal outgrowth of CTB-labelled neurons was dose-dependently stimulated with a maximal effect of 50% at 10(-10) M alpha-MSH. The maximal effect for stimulation of axon outgrowth for the total cortex population was observed at 10(-8) M alpha-MSH. In addition dendrite outgrowth of both total and CTB-labelled neurons is stimulated in a dose-dependent manner with maximal effects (varying between 46 and 48%) at 10(-8) M alpha-MSH. Explanations in the shift for the optimal alpha-MSH concentration for stimulation of axonal outgrowth of CTB-labelled layer V neurons as compared to total cortex neurons are discussed.
http://hdl.handle.net/2268/7202

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Brain Research 736 (1996) 91-98 - Joosten - alpha MSH.pdfPublisher postprint1.55 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.