Reference : Different mechanisms are implicated in ERBB2 gene overexpression in breast and in oth...
Scientific journals : Article
Human health sciences : Oncology
http://hdl.handle.net/2268/71361
Different mechanisms are implicated in ERBB2 gene overexpression in breast and in other cancers
English
Vernimmen, D. [> > > >]
Guéders, Maud mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Pisvin, Sonia mailto [Centre Hospitalier Universitaire de Liège - CHU > > Centre de diagnostic moleculaire >]
Delvenne, Philippe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Winkler, Rose mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
1-Sep-2003
British Journal of Cancer
Nature Publishing Group
89
5
899-906
Yes (verified by ORBi)
International
0007-0920
London
[en] immunocytochemistry ; ERBB2 promoter ; AP-2 transcription factor ; human
[en] The ERBB2 gene is overexpressed in 30% of breast cancers and this has been correlated with poor prognosis. ERBB2 is upregulated in other cancers such as prostate, pancreas, colon and ovary. In breast cancer cells, the mechanisms leading to ERBB2 gene overexpression are increased transcription and gene amplification. In these cancers, AP-2 transcription factors are involved in ERBB2 overexpression, and AP-2 levels are correlated with p185(c-erbB-2) levels. In this work, we wanted to know if the same molecular mechanisms are responsible for the ERBB2 upregulation in non-breast cancers. We compared ERBB2 gene copy number, p185(c-erbB-2) and mRNA levels with AP-2 levels in several ovary, prostate, colon and pancreas cancer cells. A moderate expression of erbB-2 mRNA and protein were observed in some cells without gene amplification. In contrast to breast cancer cells, AP-2 factors were absent or low in some non-breast cells which did express ERBB2. It is thus likely that AP-2 is not a major player in the increased levels of erbB-2 transcripts in non-breast cancer cells. The transcriptional activity of the ERBB2 promoter in colon and ovary cancer cells was estimated using reporter vectors. The results showed that the promoter regions involved in ERBB2 gene overexpression in breast cancer cells are different from those that lead to the gene upregulation in colon and ovary cancers. In conclusion, our results indicate that different transcriptional and post-transcriptional mechanisms are responsible for the increased levels of erbB-2 transcript and protein in breast and non-breast cancer cells.
http://hdl.handle.net/2268/71361
10.1038/sj.bjc.6601200

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
89-6601200a.pdfPublisher postprint216.99 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.