Article (Scientific journals)
Interfaces between dendritic cells, other immune cells, and nerve fibres in mouse Peyer's patches: Potential sites for neuroinvasion in prion diseases
Defaweux, Valérie; Dorban, Gauthier; Demonceau, Christine et al.
2005In Microscopy Research and Technique, 66 (1), p. 1-9
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Keywords :
mouse; Peyer's patches; dendritic cells; follicular dendritic cells; nerve fibres; prion
Abstract :
[en] In this study, we examined where immune cells and nerve fibres are located in mouse Peyer's patches, with a view to identifying potential sites for neuroinvasion by prions. Special attention was paid to dendritic cells, viewed as candidate transporters of infectious prion. Double immunofluorescence labellings with anti-CD11c antibody and marker for other immune cells (B cells, T cells, follicular dendritic cells) were carried out and analysed by confocal microscopy on Peyer's patch cryosections. To reveal the extensive ganglionated networks of the myenteric and submucosal plexi and the sparse meshworks of nerve strands, we used antibodies directed against different neurofilament subunits or against glial fibrillary acidic protein. In the suprafollicular dome, dendritic cells connect, via their cytoplasmic extensions, enterocytes with M cells of the follicle-associated epithelium. They are also close to B and T cells. Nerve fibres are detected in the suprafollicular dome, notably in contact with dendritic cells. Similar connections between dendritic cells, T cells, and nerve fibres are seen in the interfollicular region. Germinal centres are not innervated; inside them dendritic cells establish contacts with follicular dendritic cells and with B cells. After immunolabelling of normal prion protein, dendritic cells of the suprafollicular dome are intensely positive labelled. (c) 2005 Wiley-Liss, Inc.
Disciplines :
Life sciences: Multidisciplinary, general & others
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Defaweux, Valérie  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Dorban, Gauthier ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Demonceau, Christine ;  Université de Liège - ULiège > Département de personne et société > Psychologie du travail et des entreprises
Piret, Julie ;  Centre Hospitalier Universitaire de Liège - CHU > HOSPITALISATION - CHIR. APP. LOCOMOT. (SN +2A)
Jolois, Olivier ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Thellin, Olivier ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Thielen, Caroline ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Antoine, Nadine ;  Université de Liège - ULiège > Département de morphologie et pathologie > Histologie
Language :
English
Title :
Interfaces between dendritic cells, other immune cells, and nerve fibres in mouse Peyer's patches: Potential sites for neuroinvasion in prion diseases
Publication date :
01 January 2005
Journal title :
Microscopy Research and Technique
ISSN :
1059-910X
eISSN :
1097-0029
Publisher :
Wiley Liss, Inc., Hoboken, United States - New Jersey
Volume :
66
Issue :
1
Pages :
1-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 February 2009

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