Article (Scientific journals)
Do Bovine Lymphocytes Express a Peculiar Prion Protein?
Mélot, France; Thielen, Caroline; Labiet, T. et al.
2002In Developmental Immunology, 9 (4), p. 245-52
Peer Reviewed verified by ORBi
 

Files


Full Text
Mélot.pdf
Author preprint (307.56 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Blotting, Western; Cattle/immunology; Cells, Cultured; Encephalopathy, Bovine Spongiform/immunology; Flow Cytometry; Glycosylation; Lymphocyte Activation; Lymphocyte Subsets/metabolism; Lymphoid Tissue/metabolism; PrPC Proteins/metabolism; Protein Isoforms/metabolism
Abstract :
[en] The cellular prion protein (PrPc) is a glycolipid-anchored cell surface protein that usually exhibits three glycosylation states. Its post-translationally modified isoform, PrPsc, is involved in the pathogenesis of various transmissible spongiform encephalopathies (TSEs). In bovine species, BSE infectivity appears to be restricted to the central nervous system; few or no detectable infectivity is found in lymphoid tissues in contrast to scrapie or variant CJD. Since expression of PrPc is a prerequisite for prion replication, we have investigated PrPc expression by bovine immune cells. Lymphocytes from blood and five different lymph organs were isolated from the same animal to assess intra- and interindividual variability of PrPc expression, considering six individuals. As shown by flow cytometry, this expression is absent or weak on granulocytes but is measurable on monocytes, B and T cells from blood and lymph organs. The activation of the bovine cells produces an upregulation of PrPc. The results of our in vitro study of PrPc biosynthesis are consistent with previous studies in other species. Interestingly, western blotting experiments showed only one form of the protein, the diglycosylated band. We propose that the glycosylation state could explain the lack of infectivity of the bovine immune cells.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Veterinary medicine & animal health
Author, co-author :
Mélot, France ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine)
Thielen, Caroline ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Labiet, T.
Eisher, S.
Jolois, Olivier ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Antoine, Nadine ;  Université de Liège - ULiège > Département de morphologie et pathologie > Histologie
Language :
English
Title :
Do Bovine Lymphocytes Express a Peculiar Prion Protein?
Publication date :
December 2002
Journal title :
Developmental Immunology
ISSN :
1044-6672
Publisher :
Hindawi Publishing Corporation
Volume :
9
Issue :
4
Pages :
245-52
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 February 2009

Statistics


Number of views
92 (11 by ULiège)
Number of downloads
5 (5 by ULiège)

Scopus citations®
 
2
Scopus citations®
without self-citations
1
OpenCitations
 
2

Bibliography


Similar publications



Contact ORBi