Reference : Biological characterization of bovine herpesvirus 1 recombinants possessing the vacci...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Life sciences : Microbiology
http://hdl.handle.net/2268/7129
Biological characterization of bovine herpesvirus 1 recombinants possessing the vaccine glycoprotein E negative phenotype
English
Muylkens, Benoît mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
Meurens, F. [> > > >]
Schynts, F. [> > > >]
de Fays, K. [> > > >]
Pourchet, A. [> > > >]
Thiry, Julien mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Antoine, Nadine mailto [Université de Liège - ULg > Département de morphologie et pathologie > Histologie >]
Thiry, Etienne mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
31-Mar-2006
Veterinary Microbiology
Elsevier Science Bv
113
3-4
283-291
Yes (verified by ORBi)
International
0378-1135
Amsterdam
[en] bovine herpesvirus 1 ; recombination ; glycoprotein E ; marker vaccine
[en] Intramolecular recombination is a frequent event during the replication cycle of bovine herpesvirus 1 (BoHV-1). Recombinant viruses frequently arise and survive in cattle after concomitant nasal infections with two BoHV-1 mutants. The consequences of this process, related to herpesvirus evolution, have to be assessed in the context of large use of live marker vaccines based on glycoprotein E (gE) gene deletion. In natural conditions, double nasal infections by vaccine and wild-type strains are likely to occur. This situation might generate virulent recombinant viruses inducing a serological response indistinguishable from the vaccine one. This question was addressed by generating in vitro BoHV-1 recombinants deleted in the gE gene from seven wild-type BoHV-1 strains and one mutant strain deleted in the genes encoding gC and gE. In vitro growth properties were assessed by virus production, one step growth kinetics and plaque size assay. Heterogeneity in the biological properties was shown among the investigated recombinant viruses. The results demonstrated that some recombinants. in spite of their gE minus phenotype, have biological characteristics close to wild-type BoHV-1. (c) 2005 Elsevier B.V. All rights reserved.
http://hdl.handle.net/2268/7129
10.1016/j.vetmic.2005.11.038

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