Article (Scientific journals)
BCL-3 degradation involves its polyubiquitination through a FBW7-independent pathway and its binding to the proteasome subunit PSMB1.
Keutgens, Aurore; Zhang-Shao, Xin; Shostak, Kateryna et al.
2010In Journal of Biological Chemistry, 285 (33), p. 25831–25840
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Keywords :
BCL-3; polyubiquitination; NF kappa B; proteasome
Abstract :
[en] The oncogenic protein BCL-3 activates or represses gene transcription through binding with the NF-kappaB proteins p50 and p52 and is degraded through a phospho- and GSK3-dependent pathway. However, the mechanisms underlying its degradation remain poorly understood. Yeast-two-hybrid analysis led to the identification of the proteasome subunit PSMB1 as a BCL-3-associated protein. The binding of BCL-3 to PSMB1 is required for its degradation through the proteasome. Indeed, PSMB1-depleted cells are defective in degrading polyubiquitinated BCL-3. The N-terminal part of BCL-3 includes lysines 13 and 26 required for the K48-linked polyubiquitination of BCL-3. Moreover, the E3 ligase FBW7 known to polyubiquitinate a variety of substrates phosphorylated by GSK3 is dispensable for BCL-3 degradation. Thus, our data defined an unique motif of BCL-3 that is needed for its recruitment to the proteasome and identified PSMB1 as a key protein required for the proteasome-mediated degradation of a nuclear and oncogenic IkappaB protein.
Research center :
Giga-Signal Transduction - ULiège
Disciplines :
Laboratory medicine & medical technology
Biochemistry, biophysics & molecular biology
Author, co-author :
Keutgens, Aurore ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Zhang-Shao, Xin ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Shostak, Kateryna ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Robert, Isabelle
Olivier, Sabine ;  Université de Liège - ULiège > Interface Entreprises-Université
Vanderplasschen, Alain ;  Université de Liège - ULiège > Immunologie et vaccinologie
Chapelle, Jean-Paul ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Viatour, Patrick ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Merville, Marie-Paule ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Bex, Françoise
Gothot, André ;  Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
Chariot, Alain ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Language :
English
Title :
BCL-3 degradation involves its polyubiquitination through a FBW7-independent pathway and its binding to the proteasome subunit PSMB1.
Publication date :
2010
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology, Baltimore, United States - Maryland
Volume :
285
Issue :
33
Pages :
25831–25840
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS, TELEVIE, FBC
Available on ORBi :
since 29 October 2010

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