Reference : Neutrophil myeloperoxidase measurements in plasma, laminar tissue, and skin of horses...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/7106
Neutrophil myeloperoxidase measurements in plasma, laminar tissue, and skin of horses given black walnut extract
English
Riggs, L. M. [College of Veterinary Medicine, University of Georgia, Athens GA 30602 > Department of Large Animal Medicine > > >]
Franck, Thierry mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Moore, J. N. [College of Veterinary Medicine, University of Georgia, Athens GA 30602 > Department of Large Animal Medicine > > >]
Krunkosky, T. M. [College of Veterinary Medicine, University of Georgia, Athens GA 30602 > Anatomy and Radiology > > >]
Hurley, D. J. [College of Veterinary Medicine, University of Georgia, Athens GA 30602 > Population Health > > >]
Peroni, J. F. [College of Veterinary Medicine, University of Georgia, Athens GA 30602 > Department of Large Animal Medicine > > >]
de la Rebière de Pouyade, Geoffroy mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Jan-2007
American Journal of Veterinary Research
American Veterinary Medical Association
68
1
81-86
Yes (verified by ORBi)
International
0002-9645
Schaumburg
[en] Black walnut heartwood extract ; Myeloperoxidase ; Specific immunologic extraction followed by enzymatic detection ; Matrix metalloproteinase
[en] Objective-To compare measurements of myeloperoxidase (MPO) in plasma, laminar tissues, and skin obtained from control horses and horses given black walnut heartwood extract (BWHE). Animals-22 healthy 5- to 15-year-old horses. Procedures-Horses were randomly assigned to 4 groups as follows: a control group given water (n = 5) and 3 experimental groups given BWHE (17) via nasogastric intubation Experimental groups consisted of 5, 6, and 6 horses that received BWHE and were euthanatized at 1.5, 3, and 12 hours after intubation, respectively. Control horses were euthanatized at 12 hours after intubation. Plasma samples were obtained hourly for all horses. Laminar tissue and skin from the middle region of the neck were harvested at the time of euthanasia. Plasma and tissue MPO concentrations were determined via an ELISA; tissue MPO activity was measured by use of specific immunologic extraction followed by enzymatic detection. Results-Tissues and plasma of horses receiving BWHE contained significantly higher concentrations of MPO beginning at hour 3. Laminar tissue and skin from horses in experimental groups contained significantly higher MPO activity than tissues from control horses. Concentrations and activities of MPO in skin and laminar tissues were similar over time. Conclusions and Clinical Relevance-in horses, BWHE administration causes increases in MPO concentration and activity in laminar tissue and skin and the time of increased MPO concentration correlates with emigration of WBCs from the vasculature. These findings support the hypothesis that activation of peripheral WBCs is an early step in the pathogenesis of acute laminitis.
Centre de l’Oxygène, Recherche et Développement - CORD
Supported by an American Quarter Horse Association research grant.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/7106

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