ORBi: Demol Jan - Towards a quantitative understanding of oxygen tension and cell density evolution in fibrin hydrogels

Reference : Towards a quantitative understanding of oxygen tension and cell density evolution in fib...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/70340

Title :	Towards a quantitative understanding of oxygen tension and cell density evolution in fibrin hydrogels
Language :	English
Author, co-author :	Demol, Jan [Division of Biomechanics and Engineering Design, K.U.Leuven, Celestijnenlaan 300C, 3001 Leuven, Belgium, > > > >]
	Lambrechts, Dennis [Division of Biomechanics and Engineering Design, K.U.Leuven, Celestijnenlaan 300C, 3001 Leuven, Belgium, > > > >]
	Geris, Liesbet [Université de Liège - ULg > Département d'aérospatiale et mécanique > Génie biomécanique >]
	Schrooten, Jan [Department of Metallurgy and Materials Engineering, K.U.Leuven, Kasteelpark Arenberg 44, 3001 Leuven, Belgium, > > > >]
	Van Oosterwyck, Hans [Division of Biomechanics and Engineering Design, K.U.Leuven, Celestijnenlaan 300C, 3001 Leuven, Belgium > > > >]
Publication date :	2010
Journal title :	Biomaterials
Publisher :	Butterworth Heinemann
Volume :	32
Issue/season :	1
Pages :	107-118
Audience :	International
ISSN :	0142-9612
City :	Oxford
Country :	United Kingdom
Keywords :	[en] Bone tissue engineering ; Fibrin ; Hydrogel ; In vitro test ; Modelling
Abstract :	[en] The in vitro culture of hydrogel-based constructs above a critical size is accompanied by problems of unequal cell distribution when diffusion is the primary mode of oxygen transfer. In this study, an experimentally informed mathematical model was developed to relate cell proliferation and death inside fibrin hydrogels to the local oxygen tension in a quantitative manner. The predictive capacity of the resulting model was tested by comparing its outcomes to the density, distribution and viability of human periosteum derived cells (hPDCs) that were cultured inside fibrin hydrogels in vitro. The model was able to reproduce important experimental findings, such as the formation of a multilayered cell sheet at the hydrogel periphery and the occurrence of a cell density gradient throughout the hydrogel. In addition, the model demonstrated that cell culture in fibrin hydrogels can lead to complete anoxia in the centre of the hydrogel for realistic values of oxygen diffusion and consumption. A sensitivity analysis also identified these two parameters, together with the proliferation parameters of the encapsulated cells, as the governing parameters for the occurrence of anoxia. In conclusion, this study indicates that mathematical models can help to better understand oxygen transport limitations and its influence on cell behaviour during the in vitro culture of cellseeded hydrogels.
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/70340

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