Reference : The detection and quantification of a digenean infection in the snail host with special ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Environmental sciences & ecology
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/7019
The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.
English
Caron, Yannick mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Rondelaud, Daniel [> > > >]
Losson, Bertrand mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
2008
Parasitology Research
Springer Science & Business Media B.V.
103
4
735-44
Yes (verified by ORBi)
International
0932-0113
[en] Fasciola sp. ; DNA ; Lymnaeids ; Microscopy ; PCR
[en] In this review, ten methods used to study digenean infections in their intermediate hosts were compared to determine which one should be used either in the field or in the lab to establish the prevalence and intensity of infections in snails. Snail crushing and snail dissection allow quick establishing of prevalence in natural or experimental infections, whereas histology is considered as the most accurate approach to assess the intensity of infection. The follow-up of cercarial shedding only gave an idea on cercarial production. Among recently developed techniques, polymerase chain reaction (PCR) brings the most accurate information and shows high sensitivity and specificity levels when compared to blotting techniques. The easiness and relatively low cost of the basic PCR protocol make it interesting to investigate the epidemiology of the liver fluke in a lab with limited financial resources. Nevertheless, if this technique allows a relatively good estimation of the prevalence, information concerning the intensity of infection is best obtained through real time PCR. However, at the time being this technique is too expensive to be used routinely in the field. The choice between classical or new techniques is usually based on a compromise, as each technique has its advantages and drawbacks.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/7019
10.1007/s00436-008-1086-1
The original publication is available at www.springerlink.com
Springer Verlag is acknowledged

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