Reference : Temporal and spatial expression of tumor-associated antigen RCAS1 in pregnant mouse uter...
Scientific journals : Article
Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
Temporal and spatial expression of tumor-associated antigen RCAS1 in pregnant mouse uterus.
Tskitishvili, Ekaterine[Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement > > Osaka University Graduate School of Medicine, Department of Obstetrics and Gynecology >]
Nakamura, Hitomi[Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology > > >]
[en] PROBLEM: The tumor-associated antigen RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is considered to play a role in the inhibition of maternal immune response during pregnancy, and participates in the initiation of labor and placental detachment. The aim of our study was to investigate the expression of RCAS1 protein in the uteri of normal pregnant mice. METHOD: of study Uteri with fetuses were collected from pregnant ICR mice on days 1.5, 3.5, 5.5, 7.5, and 9.5 p.c., and uterine and placental tissues were obtained separately on days 11.5, 13.5, 15.5, and 17.5 p.c. Samples were examined using real-time (RT)-PCR, Western blotting, and immunohistochemical analyses. RESULTS: In normal pregnant mice, RCAS1 protein mRNA was significantly increased on day 7.5 p.c. Antigen localization was detected in the placenta, decidua, and fetus. CONCLUSION: The results of this study suggest the importance of day 7.5 p.c. for RCAS1 protein expression in connection with placentation as a possible target for future in vivo studies.
This is an electronic version (Author’s preprint) of the article published in American Journal of Reproductive Immunology, 2010 Feb 63(2):137-43. The original published version is available at:http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0897.2009.00772.x/pdf