[en] We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit adapted on different "open" automated platforms. We performed a comparison between the IDS EIA kit adapted on two different "open"automated platforms and the DiaSorin RIA. We found a systematic bias (higher levels with the IDS EIA kit) for concentrations more than 50-60 ng/mL that was less obvious when the IDS EIA was used in its manual procedure. We thus suggest to use the IDS EIA kit in its manual procedure rather than to adapt it on an automated platform, and to interpret cautiously a 25(OH)D greater than 100 ng/mL with this kit.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Cavalier, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Huberty, V.
Cormier, C.
Souberbielle, Jean-Claude
Language :
English
Title :
Overestimation of the 25OHD serum concentration with the automated IDS EIA kit.
Publication date :
February 2011
Journal title :
Journal of Bone and Mineral Research
ISSN :
0884-0431
eISSN :
1523-4681
Publisher :
American Society for Bone and Mineral Research, Washington, United States - District of Columbia
Souberbielle JC, Prie D, Courbebaisse M, et al. [Update on vitamin D and evaluation of vitamin D status]. Ann Endocrinol (Paris). 2008; 69: 501-510 [in French].
Cavalier E, Wallace AM, Knox S, Mistretta VI, Cormier C, Souberbielle JC., Serum vitamin D measurement may not reflect what you give to your patients. J Bone Miner Res. 2008; 23: 1864.
Adams JS, Hewison M., Update in vitamin D. J Clin Endocrinol Metab. 2010; 95: 471-4478.
Bischoff-Ferrari HA, Giovannucci E, Willett WC, Dietrich T, Dawson-Hughes B., Estimation of optimal serum concentrations of 25-hydroxyvitamin D for multiple health outcomes. Am J Clin Nutr. 2006; 84: 18-28.
Dawson-Hughes B, Heaney RP, Holick MF, Lips P, Meunier PJ, Vieth R., Estimates of optimal vitamin D status. Osteoporos Int. 2005; 16: 713-716.
Holick MF., Vitamin D deficiency. N Engl J Med. 2007; 357: 266-281.
Hollis BW., Circulating 25-hydroxyvitamin D levels indicative of vitamin D sufficiency: implications for establishing a new effective dietary intake recommendation for vitamin D. J Nutr. 2005; 135: 317-322.
Jean G, Souberbielle JC, Chazot C., Monthly cholecalciferol administration in haemodialysis patients: a simple and efficient strategy for vitamin D supplementation. Nephrol Dial Transplant. 2009; 24: 3799-3805.
Przybelski R, Agrawal S, Krueger D, Engelke JA, Walbrun F, Binkley N., Rapid correction of low vitamin D status in nursing home residents. Osteoporos Int. 2008; 19: 1621-1628.
Hypponen E, Turner S, Cumberland P, Power C, Gibb I., Serum 25-hydroxyvitamin D measurement in a large population survey with statistical harmonization of assay variation to an international standard. J Clin Endocrinol Metab. 2007; 92: 4615-4622.
Kimball SM, Vieth R., A comparison of automated methods for the quantitation of serum 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D. Clin Biochem. 2007; 40: 1305-1310.
Roth HJ, Schmidt-Gayk H, Weber H, Niederau C., Accuracy and clinical implications of seven 25-hydroxyvitamin D methods compared with liquid chromatography-tandem mass spectrometry as a reference. Ann Clin Biochem. 2008; 45: 153-159.