Reference : Overestimation of the 25OHD serum concentration with the automated IDS EIA kit.
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
http://hdl.handle.net/2268/69506
Overestimation of the 25OHD serum concentration with the automated IDS EIA kit.
English
Cavalier, Etienne mailto [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
Huberty, V. [> > > >]
Cormier, C. [> > > >]
Souberbielle, J. C. [> > > >]
Feb-2011
Journal of Bone and Mineral Research
American Society for Bone and Mineral Research
26
2
434-6
Yes (verified by ORBi)
International
0884-0431
1523-4681
Washington
DC
[en] vitamin D ; analytical ; overestimation
[en] We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit adapted on different "open" automated platforms. We performed a comparison between the IDS EIA kit adapted on two different "open"automated platforms and the DiaSorin RIA. We found a systematic bias (higher levels with the IDS EIA kit) for concentrations more than 50-60 ng/mL that was less obvious when the IDS EIA was used in its manual procedure. We thus suggest to use the IDS EIA kit in its manual procedure rather than to adapt it on an automated platform, and to interpret cautiously a 25(OH)D greater than 100 ng/mL with this kit.
http://hdl.handle.net/2268/69506
10.1002/jbmr.190

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