Reference : UPLC MS/MS method for the determination of bupropion and its main metabolites in huma...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
UPLC MS/MS method for the determination of bupropion and its main metabolites in human whole blood
Denooz, Raphael mailto [Université de Liège - ULg > > Toxicologie clinique >]
Mercerolle, M. [ > > ]
Lachatre, C. [ > > ]
Charlier, Corinne mailto [Université de Liège - ULg > Département de pharmacie > Chimie toxicologique >]
Journal of Analytical Toxicology
Preston Publications Inc
Yes (verified by ORBi)
[en] A selective and sensitive ultra-performance liquid chromatography (UPLC)–electrospray ionization-tandem mass spectrometry (MS) method for simultaneous determination of bupropion and its main metabolites, hydroxybupropion, erythrohydrobupropion, and threohydrobupropion, in human whole blood is presented. The sample preparation consists of cleanup protein precipitation with methanol combined with a solid-phase extraction on Oasis HLB cartridges. Analytes were separated on a Waters Acquity UPLC® BEH phenyl column using a binary mobile phase consisting of ammonium formate buffer (2 mM, pH 4) and acetonitrile. Detection was performed on a Waters Acquity UPLC system coupled to a Quattro Premier triple-quadrupole MS in positive ion selected reaction monitoring. Internal standards were bupropion-d9 and hydroxybupropion-d6. Linearity was from 5 to 1000 ng/mL for bupropion and from 10 to 2000 ng/mL for metabolites. Accuracy profiles (80–120%), precision (< 15%), and limits of detection (1 ng/mL for bupropion and 2 ng/mL for metabolites) were also evaluated and responded to all criteria of validation. The aim of this study was to compare this presented method with a previously described method developed on a classic liquid chromatography–tandem MS system.

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