Reference : Generic systems for the enantioseparation of basic drugs in NACE using single-isomer ...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/67943
Generic systems for the enantioseparation of basic drugs in NACE using single-isomer anionic CDs
English
Rousseau, Anne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Gillotin, Florian [> >]
Chiap, Patrice mailto [Université de Liège - ULg > > Pharmacologie clinique >]
Bodoki, Ede [> >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
2011
Journal of Pharmaceutical & Biomedical Analysis
Elsevier Science
54
154-159
Yes (verified by ORBi)
International
0731-7085
Oxford
United Kingdom
[en] Nonaqueous capillary electrophoresis ; Optimization ; Basic drugs ; Single-isomer anionic cyclodextrin ; In vitro metabolites
[en] The enantioseparation of ten basic drugs was evaluated in NACE systems using heptakis(2-O-methyl-3-O-acetyl-6-O-sulfo)-β-CD (HMAS-β-CD). For this purpose, a D-optimal design with 21 experimental points was applied. Four antifungal agents (econazole, isoconazole, miconazole, sulconazole), three local anesthetics (bupivacaine, mepivacaine and prilocaine), two sympathomimetics (salbutamol and terbutaline) and one β-blocker (carvedilol) were selected as basic model analytes. The influence on the enantiomeric resolution of anionic CD and BGE anion concentrations as well as the BGE anion nature was investigated. For all studied analytes, the enantiomeric resolution was shown to be significantly influenced by the CD concentration. Based on the observed results, a generic NACE system was recommended, namely 20 mM HMAS-β-CD and 10 mM ammonium camphorSO3- in methanol acidified with 0.75 M formic acid. Moreover, this NACE system was compared to previous conditions with heptakis(2,3-di-O-methyl-6-O-sulfo)-β-CD (HDMS-β-CD) or heptakis(2,3-di-O-acetyl-6-O-sulfo)-β-CD (HDAS-β-CD). Finally, two generic systems using either HDAS-β-CD or HMAS-β-CD were proposed and evaluated for the enantioseparation of ketamine and norketamine after incubation of ketamine in phenobarbital-induced male rat liver microsomes systems.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
http://hdl.handle.net/2268/67943

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