Article (Scientific journals)
Generic systems for the enantioseparation of basic drugs in NACE using single-isomer anionic CDs
Rousseau, Anne; Gillotin, Florian; Chiap, Patrice et al.
2011In Journal of Pharmaceutical and Biomedical Analysis, 54, p. 154-159
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Keywords :
Nonaqueous capillary electrophoresis; Optimization; Basic drugs; Single-isomer anionic cyclodextrin; In vitro metabolites
Abstract :
[en] The enantioseparation of ten basic drugs was evaluated in NACE systems using heptakis(2-O-methyl-3-O-acetyl-6-O-sulfo)-β-CD (HMAS-β-CD). For this purpose, a D-optimal design with 21 experimental points was applied. Four antifungal agents (econazole, isoconazole, miconazole, sulconazole), three local anesthetics (bupivacaine, mepivacaine and prilocaine), two sympathomimetics (salbutamol and terbutaline) and one β-blocker (carvedilol) were selected as basic model analytes. The influence on the enantiomeric resolution of anionic CD and BGE anion concentrations as well as the BGE anion nature was investigated. For all studied analytes, the enantiomeric resolution was shown to be significantly influenced by the CD concentration. Based on the observed results, a generic NACE system was recommended, namely 20 mM HMAS-β-CD and 10 mM ammonium camphorSO3- in methanol acidified with 0.75 M formic acid. Moreover, this NACE system was compared to previous conditions with heptakis(2,3-di-O-methyl-6-O-sulfo)-β-CD (HDMS-β-CD) or heptakis(2,3-di-O-acetyl-6-O-sulfo)-β-CD (HDAS-β-CD). Finally, two generic systems using either HDAS-β-CD or HMAS-β-CD were proposed and evaluated for the enantioseparation of ketamine and norketamine after incubation of ketamine in phenobarbital-induced male rat liver microsomes systems.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Rousseau, Anne ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Gillotin, Florian
Chiap, Patrice ;  Université de Liège - ULiège > Pharmacologie clinique
Bodoki, Ede
Crommen, Jacques ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Fillet, Marianne ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Servais, Anne-Catherine  ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Generic systems for the enantioseparation of basic drugs in NACE using single-isomer anionic CDs
Publication date :
2011
Journal title :
Journal of Pharmaceutical and Biomedical Analysis
ISSN :
0731-7085
eISSN :
1873-264X
Publisher :
Elsevier Science, Oxford, United Kingdom
Volume :
54
Pages :
154-159
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Fonds Léon Fredericq [BE]
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