Article (Scientific journals)
Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-alpha in endobronchial biopsies in horses with recurrent airway obstruction.
Riihimäki, Miia; Raine, Amanda; Pourazar, Jamshid et al.
2008In BMC Veterinary Research, 4, p. 8
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Keywords :
Animals; Biopsy/veterinary; Bronchi/cytology/metabolism; Epithelial Cells/metabolism; Gene Expression Regulation; Horse Diseases/genetics/metabolism; Horses; Interleukin-1/genetics/metabolism; Interleukin-10/genetics/metabolism; Lung Diseases, Obstructive/genetics/metabolism/veterinary; RNA, Messenger/genetics/metabolism; Respiratory Mucosa/cytology/metabolism; Tumor Necrosis Factor-alpha/genetics/metabolism
Abstract :
[en] BACKGROUND: The aim of this study was to evaluate the contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of cytokines of innate immunity IL-6, IL-10 and TNF-alpha, in horses with Recurrent Airway Obstruction (RAO) during exacerbation and in remission. RESULTS: Despite marked clinical and physiologic alterations between exacerbation and after remission in the RAO horses no differences were detected in either cytokine mRNA or protein levels. Moreover, the expression of investigated cytokines in RAO horses on pasture did not differ from controls.In comparing real-time PCR analysis to results of immunohistochemistry only IL-10 mRNA and protein levels in RAO horses on pasture were significantly correlated (rs = 0.893, p = 0.007). Curiously, in controls examined on pasture the TNF-alpha protein level was positively correlated to IL-10 mRNA expression (rs = 0.967, p = 0.007) and negatively correlated to IL-6 mRNA expression (rs = -0.971, p = 0.001). CONCLUSION: Given the complementary relationship of assessing cytokines directly by immunohistochemistry, or indirectly by PCR to mRNA, the lack of significant changes in either mRNA or protein levels of IL-6, IL-10 or TNF-alpha mRNA in RAO horses in exacerbation suggests that these particular cytokines in bronchial tissue may not play a substantive role in the active inflammation of this disease. To support this contention further studies examining time dependency of expression of IL-6, IL-10 or TNF-alpha are needed, as is expansion of the range of cytokines to include other key regulators of airway inflammation.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Riihimäki, Miia;  Swedish University of Agricultural Sciences - SLU > Clinical sciences, Equine internal medicine
Raine, Amanda;  Biomedical Centre Uppsala, Sweden > Cell and Molecular biology
Pourazar, Jamshid;  University Hospital, Umea, Sweden > Respiratory Medicine and Allergy
Sandström, Thomas;  University Hospital, Umea, Sweden > Respiratory Medicine and Allergy
Art, Tatiana ;  Université de Liège - ULiège > Faculty of Veterinary Medicine, Equine Sports Medicine Centre
Lekeux, Pierre ;  Université de Liège - ULiège > Faculty of Veterinary Medicine, Equine Sports Medicine Centre
Couëtil, Laurent;  Purdue University, West Lafayette, IN, USA > Veterinary clinical sciences, School of veterinary medicine
Pringle, John;  Swedish University of Agricultural Sciences - SLU > Clinical sciences, Equine internal medicine
Language :
English
Title :
Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-alpha in endobronchial biopsies in horses with recurrent airway obstruction.
Publication date :
2008
Journal title :
BMC Veterinary Research
eISSN :
1746-6148
Publisher :
BioMed Central
Volume :
4
Pages :
8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 February 2009

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