Article (Scientific journals)
ESR technique for non invasive way to quantify cyclodextrins effect on cell membranes
Grammenos, Angeliki; Mouithys-Mickalad, Ange; Guelluy, Pierre-Henri et al.
2010In Biochemical and Biophysical Research Communications, p. 1-5
Peer reviewed
 

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Keywords :
cholesterol; cyclodextrin; ESR
Abstract :
[en] A new way to study the action of cyclodextrin was developed to quantify the damage caused on cell membrane and lipid bilayer. The Electron Spin Resonance (ESR) spectroscopy was used to study the action of Randomly methylated-beta-cyclodextrin (Rameb) on living cells (HCT-116). The relative anisotropy observed in ESR spectrum of nitroxide spin probe (5-DSA and cholestane) is directly related to the rotational mobility of the probe,which can be further correlated with themicroviscosity. The use of ESR probes clearly shows a close correlation between cholesterol contained in cells and cellular membrane microviscosity. This study also demonstrates the Rameb ability to extract cholesterol and phospholipids in time- and dose-dependent ways. In addition, ESR spectra enabled to establish that cholesterol is extracted from lipid rafts to form stable aggregates. The present work supports that ESR is an easy, reproducible and noninvasive technique to study the effect of cyclodextrins on cell membranes.
Disciplines :
Physics
Author, co-author :
Grammenos, Angeliki ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Mouithys-Mickalad, Ange ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Guelluy, Pierre-Henri ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Lismont, Marjorie ;  Université de Liège - ULiège > Département de physique > Biophotonique
Piel, Géraldine ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Hoebeke, Maryse  ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Language :
English
Title :
ESR technique for non invasive way to quantify cyclodextrins effect on cell membranes
Publication date :
24 June 2010
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press, San Diego, United States - California
Pages :
1-5
Peer reviewed :
Peer reviewed
Available on ORBi :
since 30 July 2010

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