Reference : ESR technique for non invasive way to quantify cyclodextrins effect on cell membranes
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Physics
http://hdl.handle.net/2268/67428
ESR technique for non invasive way to quantify cyclodextrins effect on cell membranes
English
Grammenos, Angeliki mailto [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
Mouithys-Mickalad, Ange mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Guelluy, Pierre-Henri mailto [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
Lismont, Marjorie mailto [Université de Liège - ULg > Département de physique > Biophotonique >]
Piel, Géraldine mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Hoebeke, Maryse mailto [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
24-Jun-2010
Biochemical and Biophysical Research Communications
Academic Press
1-5
Yes (verified by ORBi)
International
0006-291X
1090-2104
San Diego
CA
[en] cholesterol ; cyclodextrin ; ESR
[en] A new way to study the action of cyclodextrin was developed to quantify the damage caused on cell membrane and lipid bilayer. The Electron Spin Resonance (ESR) spectroscopy was used to study the action of Randomly methylated-beta-cyclodextrin (Rameb) on living cells (HCT-116). The relative anisotropy observed in ESR spectrum of nitroxide spin probe (5-DSA and cholestane) is directly related to the rotational mobility of the probe,which can be further correlated with themicroviscosity. The use of ESR probes clearly shows a close correlation between cholesterol contained in cells and cellular membrane microviscosity. This study also demonstrates the Rameb ability to extract cholesterol and phospholipids in time- and dose-dependent ways. In addition, ESR spectra enabled to establish that cholesterol is extracted from lipid rafts to form stable aggregates. The present work supports that ESR is an easy, reproducible and noninvasive technique to study the effect of cyclodextrins on cell membranes.
Researchers ; Professionals
http://hdl.handle.net/2268/67428
10.1016/j.bbrc.2010.06.050

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