Reference : Inhibition of Paf-Induced Platelet Aggregation by Web 2086 'in-Vitro', an Antagonist ...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/6713
Inhibition of Paf-Induced Platelet Aggregation by Web 2086 'in-Vitro', an Antagonist to the Receptor for Platelet-Activating Factor, in Bovine
English
Bastos da Silva, Miriam [Université de Liège - ULG > Faculty of Veterinary Medicine > Laboratory for Functional Investigation > >]
Herion, Francine mailto [Centre Hospitalier Universitaire de Liège - CHU > Thrombosis Hemostasis Unit > > >]
Raskinet, Renée [Centre Hospitalier Universitaire de Liège - CHU > Thrombosis Hemostasis Unit > > >]
David, Jean-Louis [Université de Liège - ULg > Thrombosis Hemostasis Unit > > >]
Gustin, Pascal mailto [Université de Liège - ULg > Pharmacology and Toxicology > > >]
Doizé, Cécile [Université de Liège - ULg > Faculty of Veterinary Medicine > Histology > >]
Lekeux, Pierre mailto [Université de Liège - ULg > Faculty of Veterinary Medicine > Laboratory for Functional Investigation > >]
1996
Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine
Blackwell Publishing
43
7
399-413
Yes (verified by ORBi)
International
0931-184X
1439-0442
Berlin
Germany
[en] The sensitivity of bovine platelet aggregation in response to PAF stimulation and the ability of WEB 2086 (a thieno-triazolodiazepine) to inhibit response to PAF-induced platelet aggregation were investigated in the blood from five healthy male Belgian Blue calves. The recorded response to PAF showed a plateau which was dependent on the PAF concentration. Platelet aggregation induced by PAF consists of two mechanisms: reversible and irreversible aggregations which are accompanied by the release of platelet granule contents. Reversible aggregation occurred above (2 . 10(-9) mol/l) PAF, and irreversible aggregation occurred above (2 . 10(-7) mol/l) PAF. Addition of WEB 2086 to bovine platelets in vitro induced a rightward shift in the dose-response curve to PAF. WEB 2086 inhibited PAF-induced aggregation in a competitive reversible manner (pA2 = 7.61). The results of our study show that PAF induces platelet aggregation in platelet-rich plasma (PRP) and that addition of WEB 2086 to bovine platelets in vitro inhibits PAF-induced Platelet Aggregation.
Bastos is a recipient of a grant from CNPq and FCAP Belém-Parà Brazil
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/6713

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