Reference : Analysis of tridimensional mixed cultures of mouse B16 melanoma cells and 3T3 fibroblasts.
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/670
Analysis of tridimensional mixed cultures of mouse B16 melanoma cells and 3T3 fibroblasts.
English
De Pauw, Marie-Claire mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Christiane, Y. [> >]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique >]
Bassleer, R. J. [> >]
1988
Anticancer Research
JG Delinassios Anticancer Research
8
1
153-60
Yes (verified by ORBi)
International
0250-7005
Athens
Greece
[en] The interactions between invasive malignant cells and normal fibroblastic cells were studied in a cellular spheroid model in vitro. Murine B16 melanoma cells (previously cultured in monolayer for a short period) and 3T3 mouse fibroblasts (greater than or equal to 130 passages in monolayers) were cultured under tridimensional conditions (pure or mixed spheroids). As compared to pure 3T3 or mixed spheroids, B16 spheroids were smaller and characterized by a higher proliferation rate, a lower degree of necrosis, and a less abundant extracellular matrix. Disintegration was observed in some pure 3T3 or mixed spheroids. Melanogenesis progressively increased inside B16 or mixed spheroids. By immunohistochemical methods and electron microscopy, laminin, fibronectin and collagen I, III and IV in extracellular matrix were studied in the three types of spheroids.
http://hdl.handle.net/2268/670
also: http://hdl.handle.net/2268/89544

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