ORBi: Mazzucchelli Gabriel - Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of proteins for cytosolic subproteome analysis

Reference : Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of pro...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/6696

Title :	Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of proteins for cytosolic subproteome analysis
Language :	English
Author, co-author :	Mazzucchelli, Gabriel [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
	Cellier, Nicolas A [> > > >]
	Mshviladzade, Vakhtang [> > > >]
	Elias, Riad [> > > >]
	Shim, Yong-Ho [> > > >]
	Touboul, David [Institut de Chimie des Substances Naturelles > > Laboratoire de Spectrométrie de Masse > >]
	Quinton, Loïc [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	Brunelle, Alain [Institut de Chimie des Substances Naturelles > > Laboratoire de Spectrométrie de Masse > >]
	Laprevote, Olivier [Institut de Chimie des Substances Naturelles > > Laboratoire de Spectrométrie de Masse > >]
	De Pauw, Edwin [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	Gillet, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
Publication date :	2008
Journal title :	Journal of Proteome Research
Publisher :	American Chemical Society
Volume :	7
Issue/season :	4
Pages :	1683-1692
Audience :	International
ISSN :	1535-3893
e-ISSN :	1535-3907
City :	Washington
Country :	DC
Keywords :	[en] saponin ; CMC ; pores ; mel-5 ; 2D gels ; 2D-LC-MS/MS ; TOF-SIMS ; soluble biomarkers ; fractionation
Abstract :	[en] Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.
Research units :	Giga-Systems Biology and Chemical Biology
Funders :	Fonds de la Recherche Fondamentale Collective d’Initiative des Chercheurs - FRFC convention Région wallonne FEDER
Name of the research project :	• Projet Intass –Georgie-0491 (1999 –2001 G. Balansard, coordinateur) : étude de saponines.
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/6696
	also: http://hdl.handle.net/2268/10070
DOI :	10.1021/pr7006973

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