[en] Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.
Giga-Systems Biology and Chemical Biology
Fonds de la Recherche Fondamentale Collective d’Initiative des Chercheurs - FRFC convention Région wallonne FEDER
• Projet Intass –Georgie-0491 (1999 –2001 G. Balansard, coordinateur) : étude de saponines.