Reference : Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of pro...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/6696
Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of proteins for cytosolic subproteome analysis
English
Mazzucchelli, Gabriel mailto [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
Cellier, Nicolas A [> > > >]
Mshviladzade, Vakhtang [> > > >]
Elias, Riad [> > > >]
Shim, Yong-Ho [> > > >]
Touboul, David [Institut de Chimie des Substances Naturelles > > Laboratoire de Spectrométrie de Masse > >]
Quinton, Loïc mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Brunelle, Alain [Institut de Chimie des Substances Naturelles > > Laboratoire de Spectrométrie de Masse > >]
Laprevote, Olivier [Institut de Chimie des Substances Naturelles > > Laboratoire de Spectrométrie de Masse > >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Gillet, Marie-Claire mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
2008
Journal of Proteome Research
American Chemical Society
7
4
1683-1692
Yes (verified by ORBi)
International
1535-3893
1535-3907
Washington
DC
[en] saponin ; CMC ; pores ; mel-5 ; 2D gels ; 2D-LC-MS/MS ; TOF-SIMS ; soluble biomarkers ; fractionation
[en] Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.
Giga-Systems Biology and Chemical Biology
Fonds de la Recherche Fondamentale Collective d’Initiative des Chercheurs - FRFC convention Région wallonne FEDER
• Projet Intass –Georgie-0491 (1999 –2001 G. Balansard, coordinateur) : étude de saponines.
Researchers
http://hdl.handle.net/2268/6696
also: http://hdl.handle.net/2268/10070
10.1021/pr7006973

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