[en] The gene coding for the class A beta-lactamase of Citrobacter diversus has been cloned and sequenced. It contains the information for a 294-amino-acid precursor protein, including a 27-residue N-terminal signal peptide. The deduced sequence of the N-terminal portion of the mature protein is in excellent agreement with that determined by microsequencing of the protein and readily explains the pI differences observed between the naturally occurring forms I and II of the enzyme. The sequence of the mature protein exhibits a very high degree of similarity with that of the Klebsiella oxytoca class A beta-lactamase
Disciplines :
Microbiology
Author, co-author :
Perilli, Mariagrazia; University of L'Aquila, Italy
Franceschini, Nicola; University of L'Aquila, Italy
Segatore, Bernardetta; University of L'Aquila, Italy
Amicosante, Gianfranco; University of L'Aquila, Italy
Oratore, Arduino; University of L'Aquila, Italy
Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Joris, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines
Frère, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus
