Reference : The surprising diversity of clostridial hydrogenases: a comparative genomic perspective
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/66903
The surprising diversity of clostridial hydrogenases: a comparative genomic perspective
English
[fr] La diversité surprenante des hydrogénases de clostridies: une comparaison génomique
Calusinska, Magdalena mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Happe, Thomas [ > > ]
Joris, Bernard mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines >]
Wilmotte, Annick mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes >]
Jun-2010
Microbiology
Springer Science & Business Media B.V.
156
1575-1588
Yes
International
0026-2617
[en] hydrogenase ; genome sequence ; Clostridium
[en] Among the large variety of micro-organisms capable of fermentative hydrogen production, strict
anaerobes such as members of the genus Clostridium are the most widely studied. They can
produce hydrogen by a reversible reduction of protons accumulated during fermentation to
dihydrogen, a reaction which is catalysed by hydrogenases. Sequenced genomes provide
completely new insights into the diversity of clostridial hydrogenases. Building on previous
reports, we found that [FeFe] hydrogenases are not a homogeneous group of enzymes, but exist in
multiple forms with different modular structures and are especially abundant in members of the
genus Clostridium. This unusual diversity seems to support the central role of hydrogenases in
cell metabolism. In particular, the presence of multiple putative operons encoding multisubunit
[FeFe] hydrogenases highlights the fact that hydrogen metabolism is very complex in this genus. In
contrast with [FeFe] hydrogenases, their [NiFe] hydrogenase counterparts, widely represented in
other bacteria and archaea, are found in only a few clostridial species. Surprisingly, a
heteromultimeric Ech hydrogenase, known to be an energy-converting [NiFe] hydrogenase and
previously described only in methanogenic archaea and some sulfur-reducing bacteria, was found
to be encoded by the genomes of four cellulolytic strains: Clostridum cellulolyticum, Clostridum
papyrosolvens, Clostridum thermocellum and Clostridum phytofermentans.
Communauté française de Belgique - CfB
ARC project Micro-H2
Researchers ; Students
http://hdl.handle.net/2268/66903
10.1099/mic.0.032771-0
http://mic.sgmjournals.org/cgi/reprint/156/6/1575
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