[en] A chromosomal 10355-bp segment of Enterococcus hirae S185 contains nine orfs which occur in the same order as the MraW-, FtsL-, PBP3-, MraY-, MurD-, MurG-, FtsQ-, FtsA- and FtsZ-encoding genes of the division and cell wall clusters of Escherichia coli and Bacillus subtilis. The E. hirae DNA segment lacks the genes which in E. coli encode the ligases Ddl, MurC, MurE and MurF and the integral membrane protein FtsW. The encoded E. hirae and E. coli proteins share 25% to 50% identity except FtsL and FtsQ (approximately = 14% identity).
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Microbiology
Author, co-author :
Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Thamm, Iris ; Université de Liège - ULiège > Centre d'Ingéniérie des Protéines
Sapunaric, Frédéric ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Coyette, Jean; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Centre d'ingéniérie des protéines
Language :
English
Title :
The Division and Cell Wall Gene Cluster of Enterococcus Hirae S185
Publication date :
1998
Journal title :
DNA Sequence: the Journal of DNA Sequencing and Mapping
ISSN :
1042-5179
eISSN :
1029-2365
Publisher :
Taylor & Francis, London, United Kingdom
Volume :
9
Issue :
3
Pages :
149-161
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] FRFC - Fonds de la Recherche Fondamentale Collective [BE]
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J. (1990). Basic local alignment search tool, journal of Molecular Biology 215,403-410.
Ayala, J.A., Garrido, T., De Pedro, M.A. and Vicente, M. (1994). Molecular biology of bacterial septation. In Ghuysen, J.M. and Hakenbeck, R. (eds), Bacterial Cell Wall (Elsevier, Amsterdam), pp. 73-101.
Buchanan, C.E., Henriques, A.O. and Piggot, P.J. (1994). Cell wall changes during bacterial endospore formation. In Ghuysen, J.M. and Hakenbeck, R. (eds), Bacterial cell wall (Elsevier, Amsterdam), pp. 167-183.
Coyette, J., Somz', A., Briquet, J.J., Ghuysen, J.M. and Fontana, R. (1983). Function of penicillin-binding protein 3 in Streptococcus faecium. In Hakenbeck, R., Höltje, J.V. and Labischinski, H. (eds), The target of penicillin (de Gruyter W. and Co., Berlin), pp. 523-530.
Devereux, J., Haeberli, P. and Smithies, O. (1984). A compre-hensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12,387-395.
Ghuysen, J.M. (1997) Penicillin-binding proteins. Wall pepti-doglycan assembly and resistance to penicillins: facts, doubts and hopes. International Journal of Antimicrobial Agents 8,45-60.
Ghuysen, J.M., Charlier, P., Coyette, J., Duez, C, Fonze, E., Fraipont, C, Goffin, C, Joris, B. and Nguyen-Disteche, M. (1996). Penicillin and beyond: evolution, protein fold, multimodular polypeptides, and multiprotein com-plexes. Microbial Drug Resistance 2,163-175. (Pubitemid 26249921)
Harry, E.J. and Wake, R.G. (1989). Cloning and expression of a Bacillus subtilis division initiation gene for which a homolog has not been identified in another organism. Journal of Bacteriology. 171,6835-6839. (Pubitemid 20006971)
Hopwood, D.A., Bibb, M.J., Chater, K.F., Kieser, T., Bruton, C.J., Kieser, H.M., Lydiate, D.J., Smith, CP., Ward, J.M. and Schrempf, H. (1985). Genetic Manipulation of Strepto-myces. A Laboratory Manual. The John Innes Foundation, Norwich, U.K.
Kunst, F. and 150 coauthors (1997). The complete sequence of the Gram-positive bacterium Bacillus subtilis. Nature 390, 249-256.
Margolin, W., Wang, R. and Kumar, M. (1996). Isolation of an ftsZ homolog from the archaebacterium Halobacterium salinarium : implications for the evolution of FtsZ and tubulin. Journal of Bacteriology 178,1320-1327. (Pubitemid 26073388)
Pearson, W.R. and Lipman, D.J. (1988). Improved tools for biological sequence analysis. Proceedings of the National Academy of Sciences USA 85,2444-2448.
Piras, G., El Kharroubi, A., Van Beeumen, J., Coeme, E., Coy-ette, J. and Ghuysen, J.M. (1990). Characterization of an Enterococcus hirae penicillin-binding protein 3 with low penicillin affinity. Journal of Bacteriology 172, 6856-6862.
Pucci, M.J., Thanassi, J.A., Discotto, L.F., Kessler, R.E. and Dougherty, T.J. (1997). Identification and characteriza-tion of cell wall-cell division gene clusters in pathogenic Gram-positive cocci. Journal of Bacteriology 179, 5632-5635. (Pubitemid 27387644)
Silver, J. (1991). Inverse polymerase chain reaction. In McPherson, M.J., Quirke, P. and Taylor, G.R. (eds), PCR a practical approach(lRL Press at Oxford University Press), pp. 137-146.
van Heijenoort, J. (1994). Biosynthesis of the bacterial pepti-doglycan unit. In Ghuysen, J.M. and Hakenbeck, R. (eds), Bacterial Cell Wall (Elsevier, Amsterdam), pp. 39-54.
Vicente, M. and Errington, J. (1996). Structure, function and controls in microbial division. Molecular Microbiology 20, 1-7. (Pubitemid 26124786)
Wang, X. and Lutkenhaus, J. (1996). Characterization of the ftsZ gene from Mycoplasma pulmonis, an organism lack-ing a cell wall. Journal of Bacteriology 178, 2314-2319. (Pubitemid 26123496)
Yura, T., Mori, H., Nagai, T., Nagata, A. Ishihama, N., Fujita, N., Isono, K, Mizobuchi, K. and Nakata, A. (1992). Systematic sequencing of the Escherichia coli genome : analysis of the 0 - 2.4 min region. Nucleic Acids Research 20, 3305-3308.
