Reference : Studies on the primary structures of the exocellular D-Alanyl-D-Alanine peptidases of...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/66863
Studies on the primary structures of the exocellular D-Alanyl-D-Alanine peptidases of Streptomyces strain R61 and Actinomadura strain R39
English
Duez, Colette mailto [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > >]
Frère, Jean-Marie mailto [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > >]
Ghuysen, Jean-Marie [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > > >]
Van Beeumen, Jozef [Rijksuniversiteit Gent Belgium - RUG > > Laboratorium voor Microbiologie > >]
Vandekerckhove, Joël [Rijksuniversiteit Gent Belgium - RUG > > Laboratorium voor Histologie > > >]
1981
Biochimica et Biophysica Acta - General Subjects
Elsevier Science
671
109-116
Yes (verified by ORBi)
International
0304-4165
Amsterdam
[en] DD-peptidases ; strucures ; R61, R39 ; alanyl-D-alanine peptidase ; beta-Lactam antibiotic ; Penicilin action ; (Streptomyces)
[en] The Mr 37 000 D-alanyl-D-alanine peptidase excreted by Streptomyces R61 and the Mr 53 000 D-alanyl-D-alanine peptidase excreted by Actinomadura R39 are both characterized by a very uneven distribution of the basic (Arg + Lys) amino acid residues. Trypsin degradation of the heat-denatured enzymes generates (1) thirteen soluble peptides which contain from 2 to 28 residues in the case of the R61 enzyme and nineteen soluble peptides which contain 2 to 39 residues in the case of the R39 enzyme; and (2) three large segments or core peptides which, irrespective of the enzymes from which they originate, consist of 50-60, 70-80 and 110-120 residues. About 90% of the basic (Arg + Lys) amino acid residues are recovered in the soluble tryptic peptides. The core peptides represent 62% (Mr approximately 23 000) and 45% (Mr approximately 24 000) of the untreated R61 and R39 enzymes, respectively. One 28-residue soluble peptide isolated from the R61 enzyme represents the N-terminal portion of the protein whose sequence has been established. The penicillin attachment site of the R61 enzyme has been located in one of the core peptides. For the R39 enzyme, indirect evidence shows that the penicillin binding site is probably within one of the soluble peptides.
National Institutes of Health - NIH ; Fonds de la Recherche Scientifique Médicale - FRSM
Researchers ; Professionals
http://hdl.handle.net/2268/66863

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