Reference : Cloning, Nucleotide Sequence and Amplified Expression of the Gene Encoding the Extracell...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/2268/66804

Title :	Cloning, Nucleotide Sequence and Amplified Expression of the Gene Encoding the Extracellular Metallo (Zn) Dd-Peptidase of Streptomyces Albus G
Language :	English
Author, co-author :	Duez, Colette [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
	Lakaye, Bernard [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
	Houba, Simone [> > > >]
	Dusart, Jean [> > > >]
	Ghuysen, Jean-Marie [Université de Liège - ULg > Service de microbiologie]
Publication date :	1-Sep-1990
Journal title :	FEMS Microbiology Letters
Volume :	71
Issue/season :	1-2
Pages :	215-219
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0378-1097
Keywords :	[en] Zn DD-peptidase ; Peptidoglycan ; streptomyces albus G
Abstract :	[en] The gene encoding the extracellular metallo (Zn) DD-peptidase of Streptomyces albus G has been cloned in Escherichia coli DH5 alpha MCR via pBR322 or 325, and then transferred into Streptomyces lividans TK24 via pIJ486, with substantial amplification of the expressed DD-peptidase. The gene has the information for the synthesis of a 255 amino acid precursor, the amino terminal region of which has the characteristic features of a signal peptide. The primary structure as deduced from nucleotide sequencing confirms that previously determined by chemical methods except for the occurrence of an Asp instead of Asn at position 1 and an additional Ala immediately downstream of Pro67.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/66804

	File(s) associated to this reference
	Fulltext file(s): File Commentary Version Size Access Open access Duez_1990_FEMS_215.pdf No commentary Publisher postprint 376.4 kB View/Open

All documents in ORBi are protected by a user license.

 

© ULg Library Network |  Feedback |  ORBi legal notices

Dspace v1.5