|Reference : Metabolism of no-carrier-added 2-[18F]fluoro-L-tyrosine in rats|
|Scientific journals : Article|
|Life sciences : Multidisciplinary, general & others|
|Metabolism of no-carrier-added 2-[18F]fluoro-L-tyrosine in rats|
|Aerts, Joël [Université de Liège - ULg > > Centre de recherches du cyclotron >]|
|Plenevaux, Alain [Université de Liège - ULg > > Centre de recherches du cyclotron >]|
|Lemaire, Christian [Université de Liège - ULg > > Centre de recherches du cyclotron >]|
|Giacomelli, Fabrice [Université de Liège - ULg > > Centre de recherches du cyclotron >]|
|Warnock, Geoffrey [Université de Liège - ULg > > Centre de recherches du cyclotron >]|
|Phillips, Christophe [Université de Liège - ULg > > Centre de recherches du cyclotron >]|
|Luxen, André [Université de Liège - ULg > Département de chimie (sciences) > Chimie organique de synthèse - Centre de recherches du cyclotron >]|
|BMC Medical Physics|
|[en] Background: Several fluorine-18 labelled fluoroamino acids have been evaluated as tracers for the quantitative assessment of cerebral protein synthesis in vivo by positron emission tomography (PET). Among these, 2-[18F]fluoro-L-tyrosine (2-[18F]Tyr) has been studied in mice at a low specific activity. Its incorporation into proteins is fast and metabolism via other pathways is limited. The present in vivo study was carried out in normal awake rats using no-carrier-added 2-[18F]Tyr. Under normal physiological conditions, we have studied the incorporation into proteins and the metabolism of the tracer in different brain areas.
Methods: No-carrier-added 2-[18F]Tyr was administered to awake rats equipped with chronic
arterial and venous catheters. The time course of the plasma activity was studied by arterial blood sampling. The biodistribution of the activity in the main organs was studied at the end of the
experiment. The distribution of radioactive species in plasma and brain regions was studied by
acidic precipitation of the proteins and HPLC analysis of the supernatant.
Results: The absolute uptake of radioactivity in brain regions was homogenous. In awake rats, nocarrier-added 2-[18F]Tyr exhibits a fast and almost quantitative incorporation into the proteins
fractions of cerebellum and cortex. In striatum, this incorporation into proteins and the unchanged
fraction of the tracer detected by HPLC could be lower than in other brain regions.
Conclusion: This study confirms the potential of 2-[18F]fluoro-L-tyrosine as a tracer for the
assessment of the rate of protein synthesis by positron emission tomography. The observed
metabolism suggests a need for a correction for the appearance of metabolites, at least in plasma.
|Centre de Recherches du Cyclotron - CRC|
|Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS|
|Researchers ; Professionals ; Students|
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