ORBi: Culot C. - Structural investigation of reconstituted high density lipoproteins by scanning tunnelling microscopy

Reference : Structural investigation of reconstituted high density lipoproteins by scanning tunnelli...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/66460

Title :	Structural investigation of reconstituted high density lipoproteins by scanning tunnelling microscopy
Language :	English
Author, co-author :	Culot, C. [ > > ]
	Durant, F. [ > > ]
	Lazarescu, S. [ > > ]
	Thiry, P. A. [ > > ]
	Vanloo, B. [ > > ]
	Rosseneu, M. Y. [ > > ]
	Lins, Laurence [Université de Liège - ULg > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie moléc. et numér. >]
	Brasseur, R. [ > > ]
Publication date :	2004
Journal title :	Applied Surface Science
Publisher :	Elsevier Science
Volume :	203
Pages :	151-157
Audience :	International
ISSN :	0169-4332
City :	Amsterdam
Country :	The Netherlands
Abstract :	[en] Being able to participate in the reverse cholesterol transport (RCT), high density lipoproteins (HDL) are known to be anti-atherogenic. In order to understand such a process, it is thus essential to have a detailed knowledge of the structure and molecular organisation of HDL. Reconstituted nascent high density lipoproteins (r-HDL), consisting of synthetic phospholipids together with different apolipoproteins (apo A-I, A-IV and E), were thus analysed by scanning tunnelling microscopy (STM). Both shape and dimensions of the discoidal HDL particles measured by this technique were found in good agreement with the data available from the literature. The accuracy of the STM pictures presented in this paper enables for the first time the visualisation of the molecular organisation of such macromolecules. The arrangement of the protein as antiparallel helical segments, is consistent with the general mode of organisation of apolipoprotein/phospholipid discoidal particles previously reported.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/66460
DOI :	10.1016/j.apsusc.2004.02.064

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