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| Reference : Importance of the alternative pathway of respiration for avoidance of ROS production and... |
| Scientific congresses and symposiums : Unpublished conference | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/2268/66360 | |||
| Importance of the alternative pathway of respiration for avoidance of ROS production and for optimisation of photosynthesis in Chlamydomonas | |
| English | |
Franck, Fabrice  [Université de Liège - ULg > > Biochimie végétale >] | |
| Dinant, M. [ > > ] | |
| Cardol, P. [ > > ] | |
| Remacle, Claire [Université de Liège - ULg > Département des sciences de la vie > Génétique >] | |
| Matagne, René-Fernand [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >] | |
| Jun-2008 | |
| No | |
| No | |
| International | |
| 13th International Chlamydomonas Conference | |
| 01/06/08 | |
| Hyères-les-Palmiers | |
| France | |
| [en] alternative oxidase ; Chlamydomonas reinhardtii ; photosynthesis | |
| [en] The physiological function of the alternative pathway of respiration has been investigated by analysing two RNAi C.reinhardtii lines deprived of alternative oxidase protein (AOX1). Compared to wild-type, AOX1- lines exhibited modified growth curves and reduced maximal cell density. These differences were more pronounced at high irradiance and in nitrate-containing medium (TAP NO3) rather than in ammonium-containing medium (TAP NH4). Although the alternative pathway was inactive, respiration was not significantly altered in transgenics. Light-saturation curves of O2-evolution were only slightly modified. However, non-photochemical quenching of fluorescence (NPQ) was strongly reduced. Further analysis showed that AOX1- transgenics present a reduced ability to promote the change in energy distribution between photosystems, known as state transition. This effect, which explains low NPQ in the light, was most pronounced in high-light cells cultivated in TAP NO3 medium. Moreover, AOX1- transgenics exhibited higher levels of intracellular peroxides, which suggests that inhibition of state transition might result from higher ROS production. In support of this hypothesis, addition of millimolar-range concentrations of H2O2 to wild-type inhibited the state transition promoted by the reduction of the plastoquinone pool in darkness. | |
| Researchers | |
| http://hdl.handle.net/2268/66360 |
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