You are here:
| Reference : Hematopoietic recovery in cancer patients after transplantation of autologous peripheral... |
| Scientific journals : Article | |||
| Human health sciences : Hematology | |||
| http://hdl.handle.net/2268/6611 | |||
| Hematopoietic recovery in cancer patients after transplantation of autologous peripheral blood CD34+ cells or unmanipulated peripheral blood stem and progenitor cells. | |
| English | |
Beguin, Yves  [Centre Hospitalier Universitaire de Liège - CHU > > Hématologie clinique >] | |
| Baudoux, Etienne [Centre Hospitalier Universitaire de Liège - CHU > > Thérapie cellulaire >] | |
| Sautois, Brieuc [Centre Hospitalier Universitaire de Liège - CHU > > Oncologie médicale >] | |
| Fraipont, V. [> > > >] | |
| Schaaf, Nicole [Centre Hospitalier Universitaire de Liège - CHU > > Hématologie biologique et immuno hématologie >] | |
| Pereira-Martins, Maguy [Centre Hospitalier Universitaire de Liège - CHU > > Hématologie clinique >] | |
| Paulus, Jean-Michel [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >] | |
| Sondag, Danièle [Université de Liège - ULg > Département des sciences de la santé publique > Immunohématologie - Transfusion >] | |
| Fillet, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Hématologie clinique >] | |
| 1998 | |
| Transfusion | |
| American Association of Blood Banks | |
| 38 | |
| 2 | |
| 199-208 | |
| International | |
| 0041-1132 | |
| 1537-2995 | |
| Bethesda | |
| MD | |
| [en] Antigens, CD34 ; Cell Differentiation ; Cell Division ; Hematopoiesis ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells/pathology ; Humans ; Neoplasms/therapy ; Transplantation, Autologous | |
| [en] BACKGROUND: A study of CD34+ cell selection and transplantation was carried out with particular emphasis on characteristics of short- and long-term hematopoietic recovery. STUDY DESIGN AND METHODS: Peripheral blood stem and progenitor cells (PBPCs) were collected from 32 patients, and 17 CD34+ cell-selection procedures were carried out in 15 of the 32. One patient in whom two procedures failed to provide 1 x 10(6) CD34+ cells per kg was excluded from further analysis. After conditioning, patients received CD34+ cells (n = 10, CD34 group) or unmanipulated (n = 17, PBPC group) PBPCs containing equivalent amounts of CD34+ cells or progenitors. RESULTS: The yield of CD34+ cells was 53 percent (18-100) with a purity of 63 percent (49-82). The CD34+ fraction contained 66 percent of colony-forming units--granulocyte-macrophage (CFU-GM) and 58 percent of CFU of mixed lineages, but only 33 percent of burst-forming units-erythroid (BFU-E) (p < 0.05). Early recovery of neutrophils and reticulocytes was identical in the two groups, although a slight delay in platelet recovery may be seen with CD34+ cell selection. Late hematopoietic reconstitution, up to 1.5 years after transplant, was also similar. The two groups were thus combined for analyses of dose effects. A dose of 40 x 10(4) CFU-GM per kg ensured recovery of neutrophils to a level of 1 x 10(9) per L within 11 days, 15 x 10(4) CFU of mixed lineages per kg was associated with platelet independence within 11 days, and 100 x 10(4) BFU-E per kg predicted red cell independence within 13 days. However, a continuous effect of cell dose well beyond these thresholds was apparent, at least for neutrophil recovery. CONCLUSION: CD34+ cell selection, despite lower efficiency in collecting BFU-E, provides a suitable graft with hematopoietic capacity comparable to that of unmanipulated PBPCs. In both groups, all patients will eventually show hematopoietic recovery of all three lineages with 1 x 10(6) CD34+ cells per kg or 5 x 10(4) CFU-GM per kg, but a dose of 5 x 10(6) CD34+ cells or 40 x 10(4) CFU-GM per kg is critical to ensure rapid recovery. | |
| http://hdl.handle.net/2268/6611 |
There is no file associated with this reference.
All documents in ORBi are protected by a user license.
