[en] The antimalarial properties of Tithonia diversifolia, an Asteraceae traditionally used to treat malaria, were investigated in vitro against three strains of Plasmodium falciparum. The ether extract from aerial parts of the plant collected in Sao Tome e Principe, demonstrated good antiplasmodial activity (IC 50 on FCA strain: 0.75 microg/ml). A bioassay guided fractionation of this extract led to the isolation of the known sesquiterpene lactone tagitinin C as an active component against Plasmodium (IC 50 on FCA strain: 0.33 microg/ml), but also possessing cytotoxic properties (IC 50 on HTC-116 cells: 0.706 microg/ml).
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Goffin, Eric ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Ziemons, Eric ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
De Mol, Patrick ; Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
de Madureira, Maria do Ceu; University of Coimbra - UC > Faculty of Pharmacy > Laboratory of Pharmacognosy
Martins, Ana Paula; University of Coimbra - UC > Faculty of Pharmacy > Laboratory of Pharmacognosy
da Cunha, Antonio Proenca; University of Coimbra - UC > Faculty of Pharmacy > Laboratory of Pharmacognosy
Philippe, Geneviève ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Tits, Monique ; Université de Liège - ULiège > Département de pharmacie > Phytochimie et phytothérapie
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Language :
English
Title :
In vitro antiplasmodial activity of Tithonia diversifolia and identification of its main active constituent: tagitinin C.
Publication date :
June 2002
Journal title :
Planta Medica
ISSN :
0032-0943
eISSN :
1439-0221
Publisher :
Georg Thieme Verlag Stuttgart, New York, United States - New York
Rüngeler P., Lyß G., Castro V., Pahl H.L., Merfort I. (1998) Study of three sesquiterpene lactones from Tithonia diversifolia on their anti-inflammatory activity using the transcription factor NF-κB and enzymes of the arachidonic acid pathways as targets. Planta Medica 64:588-593.
Tona L., Kambu K., Ngimbi N., Mesia K., Penge O., Lusakibanza M., Cimanga K., De Bruyne T., Apers S., Totte J., Pieters L., Vlietinck A.J. (2000) Antiamoebic and spasmolytic activities of extracts from some antidiarrhoeal traditional preparations used in Kinshasa, Congo. Phytomedicine 7:31-38.
Baruah N.C., Sharma R.P., Madhusudanan K.P., Thyagarajan G. (1979) Sesquiterpene lactones of Tithonia diversifolia. Stereochemistry of the tagitinins and related compounds. Journal of Organic Chemistry 44:1831-1835.
Pal R., Kulshreshta D.K., Rastogi R.P. (1977) Chemical constituents of Tithonia tagitiflora Desf.: Part IV - Tagitinins C, D & F. Indian Journal of Chemistry 15 B:208-211.
Trager W., Jensen J.B. (1976) Human malaria parasites in continuous cultures. Science 193:673-675.
Desjardins R., Canfield C., Haynes J.D., Chulay J. (1979) Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrobial Agents Chemotherapy 16:710-718.
Frederich M., De Pauw M.C., Llabrès G., Tits M., Hayette M.P., Brandt V., Penelle J., De Mol P., Angenot L. (2000) New antimalarial and cytotoxic sungucine derivatives from Strychnos icaja roots. Planta Medica 66:262-269.