Article (Scientific journals)
Development of a combined mechanical and enzymatic method for the isolation of intact preantral follicles from fetal, calf and adult bovine ovaries
Figueiredo, José Ricardo; Hulshof, S. C. J.; Van den Hurk, R. et al.
1993In Theriogenology, 40 (4), p. 789-799
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Keywords :
Isolation; Preantral follicles; Ovaries; Follicules préantraux; Ovaires
Abstract :
[en] The isolation of preantral follicles from the ovaries of bovine fetuses, calves and adult cows was performed using a simple, rapid mechanical and enzyme method. The ovaries were cut into small pieces with a tissue chopper. Then, the suspension was filtered successively through 500 and 100 μm nylon mesh filters. This simple mechanical procedure resulted in large numbers of isolated preantral follicles: 2,142 ± 254; 512 ± 92 and 298 ± 54 from the ovaries of bovine fetuses, calves and cows, respectively. In addition, the ovarian fragments between 100 and 500 μm were suspended in 10 ml of M199 Hepes medium plus 5% FCS and divided into 2 equal parts: one portion was used for collagenase treatment (200 U/ml) for 20 minutes, while the other served as a control. Collagenase treatment resulted in 841 ± 161; 216 ± 51 and 52 ± 17 preantral follicles from fetuses, calves and cows, respectively, compared with 312 ± 86; 52 ± 15 and 10 ± 2 in the control group. The use of collagenase with ovarian fragments selected by filtration as a method for increasing the rate of recovery of preantral follicles is described here.
Research center :
Laboratoire d'Endocrinologie et de Physiologie de la Reproduction animale
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Figueiredo, José Ricardo;  Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Hulshof, S. C. J.;  University of Utrecht > Functional Morphology
Van den Hurk, R.;  University of Utrecht > Fuctional Morphology
Ectors, Fabien  ;  Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Fontes, R. S.;  Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Nusgens, Betty ;  Université de Liège - ULiège > Laboratoire de Dermatologie expérimentale
Bevers, M. M.;  University of Utrecht > Heard Health and Reproduction
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Development of a combined mechanical and enzymatic method for the isolation of intact preantral follicles from fetal, calf and adult bovine ovaries
Publication date :
October 1993
Journal title :
Theriogenology
ISSN :
0093-691X
eISSN :
1879-3231
Publisher :
Elsevier, New York, United States - New York
Volume :
40
Issue :
4
Pages :
789-799
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
IRSIA - Institut pour l'Encouragement de la Recherche Scientifique dans l'Industrie et l'Agriculture [BE]
FRSM - Fonds de la Recherche Scientifique Médicale [BE]
Funding text :
Bourse CNPq-BIOAGRO (UFV) Brésil
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since 18 February 2009

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