Reference : In vitro identification of targeting ligands of human M cells by phage display
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
Engineering, computing & technology : Materials science & engineering
http://hdl.handle.net/2268/64811
In vitro identification of targeting ligands of human M cells by phage display
English
Fievez, V. [Université catholique de Louvain (UCL), Brussels > > Unité de Pharmacie Galénique (Bruissels), Biochimie Cellulaire, Nutritionnelle & Toxicologique (Louvain-La-Neuve) > >]
Plapied, L. [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique (Bruissels), Biochimie Cellulaire, Nutritionnelle & Toxicologique (Louvain-La-Neuve) > >]
Plaideau, C. [Université catholique de Louvain (UCL) > > Biochimie Cellulaire, Nutritionnelle & Toxicologique > >]
Legendre, D. [Université catholique de Louvain (UCL) > > Biochimie Cellulaire, Nutritionnelle & Toxicologique > >]
des Rieux, A. [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique (Bruissels), Biochimie Cellulaire, Nutritionnelle & Toxicologique (Louvain-La-Neuve) > >]
Pourcelle, V. [Université catholique de Louvain (UCL) > > Laboratoire de Chimie Médicinale > >]
Freichels, Hélène [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Jérôme, Christine mailto [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Marchand, Jacqueline [Université catholique de Louvain (UCL) > > Laboratoire de Chimie Médicinale > >]
Préat, Véronique [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique > >]
Scneider, Y. J. [Université catholique de Louvain (UCL) > > Biochimie Cellulaire, Nutritionnelle & Toxicologique > >]
15-Jul-2010
International Journal of Pharmaceutics
Elsevier Science
394
1-2
35-42
Yes (verified by ORBi)
International
0378-5173
Amsterdam
The Netherlands
[en] nanomedicine ; organic nanoparticle
[en] To improve transport of vaccine-loaded nanoparticles, the phage display technology was used to identify novel lead peptides targeting human M cells. Using an in vitro model of the human follicle-associated epithelium (FAE) which contains both Caco-2 and M cells, a T7 phage display library was screened for its ability either to bind the apical cell surface of or to undergo transcytosis across Caco-2 cells or FAE. The selection for transcytosis across both enterocytes and FAE identified three different peptide sequences (CTGKSC, PAVLG and LRVG) with high frequency. CTGKSC and LRVG sequences enhanced phage transport across M-like cells. When polymeric nanoparticles were grafted with the sequences CTGKSC and LRVG, their transport by FAE was significantly enhanced. These peptides could therefore be used to enhance the transport of vaccine-loaded nanoparticles across the intestinal mucosal barrier.
Center for Education and Research on Macromolecules (CERM)
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; The "Région Wallonne" in the frame of the "FIRST EUROPE" and "VACCINOR (WINNOMAT)" programs
Researchers
http://hdl.handle.net/2268/64811
10.1016/j.ijpharm.2010.04.023
http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T7W-4YXK4G7-4-C&_cdi=5069&_user=532038&_pii=S0378517310002954&_orig=browse&_coverDate=07%2F15%2F2010&_sk=996059998&view=c&wchp=dGLbVzz-zSkzV&md5=6b8c5d5bac1c2be6910278d6228b052a&ie=/sdarticle.pdf

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